Paola Gavazzo

Research staff

1988 Master degree in Biological Science
1993 PhD in Biophysics
1993-2001 Post doc at Biophysics Institute (IBF) CNR Genova
2001- Researcher at IBF, Genova

orcid://0000-0002-2016-4702,

Contact:

- Email paola.gavazzo@ibf.cnr.it
- Phone +39-010-6475569
- Location Genova

Research interests:

- Migraine and the voltage-gated sodium channel (Principal investigator)
- Biophysics study of stem cell differentiation (Principal investigator)
- Functional characterization of Kir4.1 and its involvement in age-related hearing loss (Principal investigator)
- Characterization of the mechanism of action of plant polyphenols from green-tea
- Volume-regulated Cl and oxidation-activated K channels in invasiveness and immunotherapy efficiency in human melanoma
- Leveraging basic knowledge of ion channel network in cancer for innovative therapeutic strategies (LIONESS)

Further collaborations:

- Familial Hemiplegic Migraine: Prof Tobias Freilinger, Klinikum Passau; Susanna Alloisio, ETT Genova
- Kir 4.1 functional characterization: Prof. Silvia Dossena, Paracelsus Medical University , Salzburg; Prof. Rossana Morabito, Dott. Alessia Remigante Università di Messina

Financial support:

- Finanziamento Telethon GGP17178 "A novel comprehensive strategy for the study of the molecular basis of Familial Hemiplegic Migraine" (Una nuova strategia integrata per lo studio delle basi molecolari dell’Emicrania Emiplegica Familiare 3)
- Finanziamento PRIN 2022YRBE8B: Functional characterization of Kir4.1 and its involvement in age-related hearing loss

Key publications:

Remigante A, Spinelli S, Zuccolini P, Gavazzo P, Marino A, Pusch M, Morabito R, Dossena S. Melatonin protects Kir2.1 function in an oxidative stress-related model of aging neuroglia. Biofactors. doi: 10.1002/biof.2024. 2023

Auffenberg E, Hedrich UB, Barbieri R, Miely D, Groschup B, Wuttke TV, Vogel N, Luhrs P, Zanardi I, Bertelli S, Spielmann N, Gailus-Durner V, Fuchs H, Hrabe de Angelis M, Pusch M, Dichgans M, Lerche H, Gavazzo P*, Plesnila N*, Freilinger T*. Hyperexcitable interneurons trigger cortical spreading depression in an Scn1a migraine model. J Clin Invest. 131(21):e142202. doi: 10.1172/JCI142202. 2021

Barbieri R, Bertelli S, Pusch M, Gavazzo P. Late sodium current blocker GS967 inhibits persistent currents induced by familial hemiplegic migraine type 3 mutations of the SCN1A gene. J Headache Pain. 20(1):107. doi: 10.1186/s10194-019-1056-2. 2019

Bertelli S, Barbieri R, Pusch M, Gavazzo P. Gain of function of sporadic/familial hemiplegic migraine-causing SCN1A mutations: Use of an optimized cDNA. Cephalalgia. doi: 10.1177/0333102418788336. 2018

Petecchia L, Sbrana F, Utzeri R, Vercellino M, Usai C, Visai L, Vassalli M, Gavazzo P. Electro-magnetic field promotes osteogenic differentiation of BM-hMSCs through a selective action on Ca(2+)-related mechanisms. Sci Rep. 14;5:13856. doi: 10.1038/srep13856. 2015

Publications (31):

BK Channel in the Physiology and in the Cancer of Pancreatic Duct: Impact and Reliability of BK Openers
Zuccolini, Paolo and Gavazzo, Paola and Pusch, Michael
frontiers in pharmacology, 13, 2022
DOI: 10.3389/fphar.2022.906608

Molecular determinants underlying volume-regulated anion channel subunit-dependent oxidation sensitivity
Bertelli S.; Zuccolini P.; Gavazzo P.; Pusch M.
journal of physiology (lond., print), 600, 2022
DOI: 10.1113/JP283321

The VRAC blocker DCPIB directly gates the BK channels and increases intracellular Ca2+ in melanoma and pancreatic duct adenocarcinoma cell lines
Zuccolini, Paolo; Ferrera, Loretta; Remigante, Alessia; Picco, Cristiana; Barbieri, Raffaella; Bertelli, Sara; Moran, Oscar; Gavazzo, Paola; Pusch, Michael
british journal of pharmacology, 2022
DOI: 10.1111/bph.15810

Mechanisms of activation of LRRC8 volume regulated anion channels
Bertelli S.; Remigante A.; Zuccolini P.; Barbieri R.; Ferrera L.; Picco C.; Gavazzo P.; Pusch M.
cellular physiology and biochemistryissn: 1015-8987s. karger, 55, 2021
DOI: 10.33594/000000329

Development of label-free biophysical markers in osteogenic maturation
Bartolozzi, Alice; Viti, Federica; De Stefano, Silvia; Sbrana, Francesca; Petecchia, Loredana; Gavazzo, Paola; Vassalli, Massimo
Journal Of The Mechanical Behavior Of Biomedical Materials, 103, 2020
DOI: 10.1016/j.jmbbm.2019.103581

Epigallocatechin-3-gallate mobilizes intracellular Ca2+ in prostate cancer cells through combined Ca2+ entry and Ca2+-induced Ca2+ release
Marchetti, C., Gavazzo, P., Burlando, B.
Life Sciences, 258, 2020
DOI: 10.1016/j.lfs.2020.118232

Gain of function of sporadic/familial hemiplegic migraine-causing SCN1A mutations: Use of an optimized cDNA
Bertelli S, Barbieri R, Pusch M, Gavazzo P
Cephalalgia, 39, 2019
DOI: 10.1177/0333102418788336

Late sodium current blocker GS967 inhibits persistent currents induced by familial hemiplegic migraine type 3 mutations of the SCN1A gene
Barbieri R.; Bertelli S.; Pusch M.; Gavazzo P.
the journal of headache and pain (testo stamp.), 20, 2019
DOI: 10.1186/s10194-019-1056-2

The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces
Bloise N, Petecchia L, Ceccarelli G, Fassina L, Usai C, Bertoglio F, Balli M, Vassalli M, Cusella De Angelis MG, Gavazzo P, Imbriani M, Visai L
PloS one, 13, 2018
DOI: 10.1371/journal.pone.0199046

A biophysical approach to quantify skeletal stem cells trans-differentiation as a model for the study of osteoporosis
Petecchia L, Viti F, Sbrana F, Vassalli M, Gavazzo P
Biophysical chemistry , 229, 2017
DOI: 10.1016/j.bpc.2017.05.011

Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation
Petecchia L, Usai C, Vassalli M, Gavazzo P
Experimental cell research, 358, 2017
DOI: 10.1016/j.yexcr.2017.06.008

Controlled single-cell cyclic compression and transcription analysis: A pilot study
Gavazzo P, Petecchia L, Facci P, Vassalli M, Viti F
Biophysical chemistry , 229, 2017
DOI: 10.1016/j.bpc.2017.07.010

Subunit-dependent oxidative stress sensitivity of LRRC8 volume-regulated anion channels
Gradogna A, Gavazzo P, Boccaccio A, Pusch M
Journal of physiology , 595, 2017
DOI: 10.1113/JP274795

The biophysics of piezo1 and piezo2 mechanosensitive channels
Soattin L.; Fiore M.; Gavazzo P.; Viti F.; Facci P.; Raiteri R.; Difato F.; Pusch M.; Vassalli M.
Biophysical chemistry, 208, 2016
DOI: 10.1016/j.bpc.2015.06.013

Electro-magnetic field promotes osteogenic differentiation of BM-hMSCs through a selective action on Ca2+-related mechanisms
Petecchia, Loredana; Sbrana, Francesca; Utzeri, Roberto; Vercellino, Marco; Usai, Cesare; Visai, Livia; Vassalli, Massimo; Gavazzo, Paola
Scientific reports , 5, 2015
DOI: 10.1038/srep13856

In vitro effect of temperature on the conformational structure and collagen binding of SdrF, a Staphylococcus epidermidis adhesin
Di Poto A.; Papi M.; Trivedi S.; Maiorana A.; Gavazzo P.; Vassalli M.; Lowy F.D.; De Spirito M.; Montanaro L.; Imbriani M.; Arciola C.R.; Visai L.
Applied microbiology and biotechnology , 99, 2015
DOI: 10.1007/s00253-015-6456-x

Elastin-Coated Biodegradable Photopolymer Scaffolds for Tissue Engineering Applications
Rossella Barenghi 1; Szabolcs Beke 2; Ilaria Romano 2; Paola Gavazzo 3; Balázs Farkas 2; Massimo Vassalli 3; Fernando Brandi 2,4; Silvia Scaglione 1
BioMed Research International (online), 2014

Multiple effects of copper on NMDA receptor currents
Marchetti, Carla; Baranowska-Bosiacka, Irena; Gavazzo, Paola
Brain research, 1542, 2014
DOI: 10.1016/j.brainres.2013.10.029

Novel ncRNAs transcribed by Pol III and elucidation of their functional relevance by biophysical approaches
Gavazzo, Paola and Vassalli, Massimo and Costa, Delfina and Pagano, Aldo
Frontiers in cellular neuroscience, 7, 2013
DOI: 10.3389/fncel.2013.00203

The P2X7 receptor as a route for non-exocytotic glutamate release: dependence on the carboxyl tail
Cervetto C; Alloisio S; Frattaroli D; Mazzotta MC; Milanese M; Gavazzo P; Passalacqua M; Nobile M; Maura G; Marcoli M
Journal of neurochemistry, 124, 2013
DOI: 10.1111/jnc.12143

Probing cytoskeleton organisation of neuroblastoma cells with single-cell force spectroscopy
Mescola A; Vella S; Scotto M; Gavazzo P; Canale C; Diaspro A; Pagano A; Vassalli M
JMR. Journal of molecular recognition, 25, 2012
DOI: 10.1002/jmr.2173

The Murine PSE/TATA-Dependent Transcriptome: Evidence of Functional Homologies with Its Human Counterpart
Bruzzone MJ; Gavazzo P; Massone S; Balbi C; Villa F; Conti A; Dieci G; Cancedda R; Pagano A
International journal of molecular sciences, 13, 2012

Acquisition of neuron-like electrophysiological properties in neuroblastoma cells by controlled expression of NDM29 ncRNA
Gavazzo P, Vella S, Marchetti C, Nizzari M, Cancedda R, Pagano A
Journal of neurochemistry, 119, 2011

Nickel modulates the electrical activity of cultured cortical neurons through a specific effect on N-methyl-d-aspartate receptor channels
Gavazzo P, Tedesco M, Chiappalone M, Zanardi I, Marchetti C
Neuroscience, 177, 2011

South African plants used in traditional medicine to treat epilepsy have an antagonistic effect on NMDA receptor currents
Marchetti C, Gavazzo P, Stafford GI, Van Staden J
Journal of ethnopharmacology, 137, 2011

The influence of Calcium ions on Nickel modulation of NMDA receptor currents
Gavazzo P, Guida P, Marchetti C
Metallomics, 3, 2011

Molecular determinants of multiple effects of Nickel on NMDA receptor channels
Gavazzo P, Guida P, Zanardi I, Marchetti C
Neurotoxicity resear, 15, 2009

Molecular determinants of Pb2+ interaction with NMDA receptor channels.
Gavazzo P.; Zanardi I.; Baranowska-Bosiacka I.; Marchetti C.
Neurochemistry international, 52, 2008
DOI: 10.1016/j.neuint.2007.07.003

Voltage-induced morphological modifications in oocyte membranes containing exogenous K(+) channels studied by electrochemical scanning force microscopy
Alessandrini A; Gavazzo P; Picco C; Facci P
Microscopy research and technique, 71, 2008
DOI: 10.1002/jemt.20552

Nickel differentially affects NMDA receptor channels in developing cultured rat neurons
Paola Gavazzo; Monica Mazzolini; Mariateresa Tedesco; Carla Marchetti
Brain research, 1078, 2006
DOI: 10.1016/j.brainres.2006.01.059

Susceptibility of insulinoma cells to Cadmium and modulation by L-type calcium channels.
Gavazzo P.; Morelli E.; Marchetti C.
BIOMETALS, 18, 2005
DOI: 10.1007/s10534-004-5789-1