Raffaella Magrassi

Research staff

She developed her thesis of degree at the Italian Tumor Institute (IST), Genova, in the laboratory of Mutagenesis where she carried out experiments of Cellular and Molecular Biology. She practiced her apprenticeship at the Biophysics Institute, National Research Council (CNR), Genova. Here she apprehended and applied the techniques of Electrophysiology and Molecular Biology on the ionic channels. During her PhD she worked in the laboratories of the Department of General and Human Physiology, at the University of Bologna where she consolidated her education in primary cell culture and Patch Clamp techniques. At the Biophysics Institute, CNR, Genova she carried out fluorometric determination of the calcium concentration and biochemical methods. At the CNR-INFM’s unit in Genoa (University of Genoa, Physics Department) her research work was focused on the characterization of nanostructured systems, with a range of applications spanning from basic research to advanced bio-medicine. During this period she improved her knowledge in Confocal and Two photon microscopy techniques in order to investigate biological system. From 2006 to February 2009 she worked as researcher on nano-medicine project (NanoMed labs) at Department of Physics, University of Genoa. The main aim of my project was to develop a system able to perform in parallel multiple planar patch clamp recordings, allowing fast screening of the effects of drugs on ion channel functionality. During these years she got acquainted with technique of soft lithography refined by Focus Ion Beam (FIB). From the 2009 she is a researcher of Institute of National Research Council (CNR). Since 2019 she co-worked with Nanobiophotonics department of Istituto italiano di tecnologia (IIT) working on a scientific projects “Quantitative single molecule localizations of channel proteins by using fluorescence super resolution tecniques” in which a quantitative super-resolution techniques is empoyied to study and characterize channel proteins heterodimer. From 2019 she works in mechanobiology lab (IBF) studing how mecahno-protein mutations affect the cell phenotypes at mechanical and physiological level .

orcid://0000-0003-3187-7764,

Contact:

- Email raffaella.magrassi@ibf.cnr.it
- Phone +39-010-6475571
- Location Genova

Website:

http://users.ge.ibf.cnr.it/smml

Research interests:

- Quantitative single molecule localizations of channel proteins by using fluorescence super resolution tecniques (Principal investigator)
- Nanomaterials (Principal investigator)
- Mechanobiology

Further collaborations:

- Collaboro dal 2011 con il dipartimento di Nanofisica dell’Istituto Italiano di Tecnologia con sede a Genova. In particolare collaboro con la Dottoressa Francesca Cella Zanacchi con cui disegno ed attuo progetti che contemplano l’analisi di campioni biologici in vivo e fissati mediante tecniche di microscopia avanzata.
- Lavoro e collaboro dal 2003 con il Dipartimento di Fisica dell’Università di Genova.

Key publications:

Abdelrasoul, Gaser N.; Magrassi, R; Dante, S; d'Amora, M; d'Abbusco, MS; Pellegrino, T; Diaspro, A . “PEGylated gold nanorods as optical trackers for biomedical applications: an in vivo and in vitro comparative study”. Nanotechnology, Volume: 27 Issue: 25, Article Number: 255101; DOI: 10.1088/0957-4484/27/25/255101; May 2016.

Jing Liu; Ranhua Xiong; Toon Brans; Saskia Lippens; Eef Parthoens; Francesca Cella Zanacchi; Raffaella Magrassi; Santosh K. Singh; Sreekumar Kurungot; Sabine Szunerits; Hannelore Marcel Ameloo; Juan C. Fraire; Eline Teirlinck; Sangram Keshari Samal; Riet De Rycke; Gaelle Houthaeve; Stefaan C. De Smedt;, Rabah Boukherroub; Kevin Braeckman. “Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy” Light: Science & Applications (2018) 7,doi: 10.1038/s41377-018-0048-3

Zanacchi, FC, Magrassi R, Diaspro, A, Manzo, C, Derr, ND, Lakadamyali, M. “Quantitative Super-Resolution Microscopy using DNA Origami” 62nd Annual Meeting of the Biophysical-Society. Biophysical Journal, vol. 114 , p. 187A-187A, ISSN: 0006-3495, FEB 2018

Magrassi, Raffaella; Ramoino, Paola; Bianchini, Paolo; Diaspro Alberto. “Protection Capabilities of Nanostructured Shells Toward Cell Encapsulation: A Saccharomyces/Paramecium Model”. Microscopy Research and Technique, volume: 73 Issue: 10 Pages: 931-936; DOI: 10.1002/jemt.20844- Jen 2010

Krol, S ; Nolte, M; Diaspro, A; Mazza, D; Magrassi, “R ; Gliozzi, A; Fery, A. “Encapsulated living cells on microstructured surfaces”. LANGMUIR Volume: 2, Issue: 2 Pages: 705-709
DOI: 10.1021/la047715q - 2005

Publications (19):

Patient's dermal fibroblasts as disease markers for visceral myopathy
Federica Viti a, Francesca Micaela Pramotton b c, Michela Martufi a g, Raffaella Magrassi a, Nicoletta Pedemonte d, Mario Nizzari a, Francesca Cella Zanacchi e, Benedetta De Michele a, Manuela Alampi f, Martina Zambito g, Giuseppe Santamaria h, Adriana Bajetto g, Sabah Sardar k, Valeria Tomati d, Paolo Gandullia i, Costanza Giampietro b c, Tullio Florio g j, Francesco Beltrame f, Massimo Vassalli k, Isabella Ceccherini h
Advanced biomaterials (Print), 2023
DOI: 10.1016/j.bioadv.2023.213355

Quantifying Protein Copy Number in Super Resolution Using an Imaging-Invariant Calibration
Cella Zanacchi F, Manzo C, Magrassi R, Derr ND, Lakadamyali M
Biophysical journal (Print), 2019
DOI: 10.1016/j.bpj.2019.04.026

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy
Liu J, Xiong RH, Brans T, Lippens S, Parthoens E, Zanacchi FC, Magrassi R, Singh SK, Kurungot S, Szunerits S, Bove H, Ameloot M, Fraire JC, Teirlinck E, Samal SK, De Rycke R, Houthaeve G, De Smedt SC, Boukherroub R, Braeckmans K
Light: Science & Applications, 2018
DOI: 10.1038/s41377-018-0048-3

PEGylated gold nanorods as optical trackers for biomedical applications: an in vivo and in vitro comparative study
Abdelrasoul GN, Magrassi R, Dante S, d'Amora M, d'Abbusco MS, Pellegrino T, Diaspro A
Nanotechnology (Bristol. Print), 2016
DOI: 10.1088/0957-4484/27/25/255101

Potential Nanotoxicity of Pegylated Gold Nanorods having Different Sizes and Shapes as a Biocompatible Contrast Agent for 2PE Imaging
Rasoul, Gaser N. Abdel[ 1,2 ] ; Magrassi, R [ 3 ] ; D'Abbusco, MS [ 4 ] ; Diaspro, A [ 1,2 ]
Biophysical journal (Print), 2013

Protection capabilities of nanostructured shells toward cell encapsulation: a saccharomyces/paramecium model
Raffaella Magrassi; Paola Ramoino; Paolo Bianchini; Alberto Diaspro
Microscopy research and technique (Print), 2010
DOI: 10.1002/jemt.20844

Nanotechnology applications in medicine
Elena Angeli; Renato Buzio,; Giuseppe Firpo; Raffaella Magrassi; Valentina Mussi; Luca Repetto; Ugo Valbusa
Tumori (Testo stamp.)), 2008

Amyloid precursor protein and presenilin1 interact with the adaptor GRB2 and modulate ERK1,2 signaling
Mario Nizzari; Valentina Venezia; Emanuela Repetto; Valentina Caorsi; Raffaella Magrassi; Maria Cristina Gagliani; Pia Carlo; Tullio Florio; Gennaro Schettini; Carlo Tacchetti; Tommaso Russo; Alberto Diaspro; Claudio Russo
The Journal of biological chemistry (Print), 2007
DOI: 10.1074/jbc.M610146200

Morphology, mechanical properties and viability of encapsulated cell
T. Svaldo-Lanero; S. Krol; R. Magrassi; A. Diaspro; R. Rolandi; A. Gliozzi; O. Cavalleri
Ultramicroscopy (Amst.), 2007
DOI: 10.1016/j.ultramic.2007.04.006

Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in bovine rod outer segments
Isabella Panfoli; Silvia Ravera; Andrea Fabiano; Raffaella Magrassi; Alberto Diaspro; Alessandro Morelli; Isidoro M. Pepe
Investigative ophthalmology & visual science (Online), 2007
DOI: 10.1167/iovs.06-0543

Protection capability of polyelectrolyte
Magrassi, R; Ramoino, P; Krol, S; Bianchini, P; Usai, C; Tagliafierro, G; Diaspro, A
Cytometry. Part A, 2006

GABAB receptor intracellular trafficking after internalization in Paramecium.
Paola Ramoino; Cesare Usai; Francesco Beltrame; Marco Fato; Lorenzo Gallus; Grazia Tagliafierro; Raffaella Magrassi; Alberto Diaspro
Microscopy research and technique (Print), 2005
DOI: 10.1002/jemt.20250

Encapsulated living cells on microstructured surfaces
Silke Krol; Marc Nolte; Alberto Diaspro; Davide Mazza; Raffaella Magrassi; Alessandra Gliozzi; Andreas Fery
Langmuir (Online), 2005
DOI: 10.1021/la047715q

Dimethyl-pepep: a DNA probe in two-photon excitation cellular imaging
Alessandro Abbotto; Giancarlo Baldini; Luca Beverina; Giuseppe Chirico; Maddalena Collini; Laura D'Alfonso; Alberto Diaspro; Raffaella Magrassi; Luca Nardo; Giorgio A. Pagani
Biophysical chemistry (Print), 2005
DOI: 10.1016/j.bpc.2004.11.004

Nanocapsules: Coating for living cells
Silke Krol; Alberto Diaspro; Raffaella Magrassi; Paola Ballario; Benedetto Grimaldi; Patrizia Filetici; Prisca Ornaghi; Paola Ramoino; Alessandra Gliozzi
IEEE transactions on nanobioscience (Online), 2004
DOI: 10.1109/TNB.2004.824279

Patch-clamp recordings in isolated sponge (Axinella polypoides) cells.
Carpaneto A., Magrassi R., Zocchi E., Cerrano C., Usai C.
Journal of biochemical and biophysical methods, 2003
DOI: 10.1016/S0165-022X(02)00184-7

ABA- and cADPR-mediated effects on respiration and filtration downstream of the temperature-signaling cascade in sponges
Zocchi, E; Basile, G; Cerrano, C; Bavestrello, G; Giovine, M; Bruzzone, S; Guida, L; Carpaneto, A; Magrassi, R; Usai, C
Journal of cell science, 2003
DOI: 10.1242/jcs.00277

Activation and inactivation of homomeric KvLQT1 potassium channels
Pusch, M; Magrassi, R; Wollnik, B; Conti, F
Biophysical journal (Print), 1998
DOI: 10.1016/S0006-3495(98)77568-X

AP endonuclease activity in humans: Development of a simple assay and analysis of ten normal individuals
Redaelli, A; Magrassi, R; Bonassi, S; Abbondandolo, A; Frosina, G
Teratogenesis, carcinogenesis, and mutagenesis (Print), 1998
DOI: 10.1002/(SICI)1520-6866(1998)18:1<17::AID-TCM3>3.0.CO;2-H