Michela Bollati

Research staff

I am a structural biologist, with experience in protein structural/functional characterization and in structural and biophysical approaches to drug design. - 2014: Researcher at IBF-CNR - 2010-2013: Post doc at Università degli Studi Di Milano - 2009: PhD in Molecular and Cellular Biology, University of Milan - 2007: Visiting Student at University of Lübeck, Germany - 2006: Master degree in Molecular Biology of the Cell cum laude at University of Milan - 2004: Bachelor in Biology at University of Milan-Bicocca

orcid://0000-0001-6579-2043,

Contact:

- Email michela.bollati@ibf.cnr.it
- Phone +39-02-50314890
- Location Milano

Website:

https://users.unimi.it/biolstru/molbd3-lab.html

Research interests:

- Structure-based drug discovery on viral proteins (Principal investigator)
- Yeaz, the resuscitation-promoting factor (Principal investigator)
- Gelsolin physiopathology
- Structural analysis of proteins involved in various human diseases and research of new therapeutic strategies

Further collaborations:

- Giovanni Bertoni (Dept Biosciences, University of Milan, Italy)
- Luisa Diomede (Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy)
- Stefano Pieraccini (Dept of Chemistry, University of Milan, Italy)
- Sara Pellegrino (Dept of Pharmaceutical Sciences, University of Milan, Italy)
- Silvia Gazzola (Dept of Science and High technology, University of Insubria, Italy)
- Oscar Burrone (International Centre for Genetic Engineering and Biotechnology, Trieste, Italy)
- Alberto Barbiroli (Dept of Food, Environmental and Nutritional Sciences, University of Milan, Italy)

Financial support:

- SAVIOR Project: Selecting AntiViral Inhibitors Of Rotavirus.
- FIRB-Futuro in Ricerca 2013-2016. Grant number: RBFR1309E (PI: Dr. Michela Bollati)

Key publications:

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion. Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M. Biochem Biophys Res Commun. 2019 Oct 8;518(1):94-99

Identification of a Small Molecule That Compromises the Structural Integrity of Viroplasms and Rotavirus Double-Layered Particles. Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, E, Ackermann M, Burrone OR, Arnoldi F. J Virol. 2018 Jan 17;92(3):e01943-17

Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa. Bollati M, Villa R, Gourlay LJ, Benedet M, Dehò G, Polissi A, Barbiroli A, Martorana AM, Sperandeo P, Bolognesi M, Nardini M. FEBS J. 2015 May;282(10):1980-97

Structures of the lamin A/C R335W and E347K mutants: implications for dilated cardiolaminopathies. Bollati M, Barbiroli A, Favalli V, Arbustini E, Charron P, Bolognesi M. Biochem Biophys Res Commun. 2012 Feb 10;418(2):217-21.

The SARS-unique domain (SUD) of SARS coronavirus contains two macrodomains that bind G-quadruplexes. Tan J, Vonrhein C, Smart OS, Bricogne G, Bollati M, Kusov Y, Hansen G, Mesters JR, Schmidt CL, Hilgenfeld R. PLoS Pathog. 2009 May;5(5):e1000428.

Publications (7):

Clinical and histopathological features of gelsolin amyloidosis associated with a novel gsn variant p.Glu580lys
Potrc M.; Volk M.; de Rosa M.; Pizem J.; Teran N.; Jaklic H.; Maver A.; Drnovsek-Olup B.; Bollati M.; Vogelnik K.; Hocevar A.; Gornik A.; Pfeifer V.; Peterlin B.; Hawlina M.; Fakin A.
International journal of molecular sciences (Print), 2021
DOI: 10.3390/ijms22031084

The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
De Rosa Matteo; Barbiroli Alberto; Bonì Francesco; Scalone Emanuele; Mattioni Davide; Vanoni Maria A.; Patrone Marco; Bollati Michela; Mastrangelo Eloise; Giorgino Toni; Milani Mario
European biophysics journal, 2020
DOI: 10.1007/s00249-019-01409-9

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion
Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M
Biochemical and biophysical research communications (Print), 2019
DOI: 10.1016/j.bbrc.2019.08.013

Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles
Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, Mastrangelo E, Ackermann M, Burrone OR, Arnoldi F
Journal of virology (Print), 2018
DOI: 10.1128/JVI.01943-17

Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa
Bollati, M. and Villa, R. and Gourlay, L.J. and Benedet, M. and Dehò, G. and Polissi, A. and Barbiroli, A. and Martorana, A.M. and Sperandeo, P. and Bolognesi, M. and Nardini, M
The FEBS journal (Print), 2015
DOI: 10.1111/febs.13254

Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
Mastrangelo, E; Bollati, M; Milani, M; Brisbarre, N; de Lamballerie, X; Coutard, B; Canard, B; Khromykh, A; Bolognesi, M
Acta crystallographica. Section F. Structural biology and crystallization communications, 2006

Preliminary characterization of (nucleoside-2 '-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses
Mastrangelo, E; Bollati, M; Milani, M; de Lamballeire, X; Brisbare, N; Dalle, K; Lantez, V; Egloff, MP; Coutard, B; Canard, B; Gould, E; Forrester, N; Bolognesi, M
Acta crystallographica. Section F. Structural biology and crystallization communications, 2006
DOI: 10.1107/S1744309106025553