Michela Bollati

Research staff

I am a structural biologist, with experience in protein structural/functional characterization and in structural and biophysical approaches to drug design. - 2014: Researcher at IBF-CNR - 2010-2013: Post doc at Università degli Studi Di Milano - 2009: PhD in Molecular and Cellular Biology, University of Milan - 2007: Visiting Student at University of Lübeck, Germany - 2006: Master degree in Molecular Biology of the Cell cum laude at University of Milan - 2004: Bachelor in Biology at University of Milan-Bicocca

orcid://0000-0001-6579-2043,

Contact:

- Email michela.bollati@ibf.cnr.it
- Phone +39-02-23699541
- Location Milano

Website:

https://users.unimi.it/biolstru/molbd3-lab.html

Research interests:

- Structure-based drug discovery on viral proteins (Principal investigator)
- Yeaz, the resuscitation-promoting factor (Principal investigator)
- Gelsolin physiopathology
- Structural analysis of proteins involved in various human diseases and research of new therapeutic strategies

Further collaborations:

- Giovanni Bertoni (Dept Biosciences, University of Milan, Italy)
- Luisa Diomede (Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy)
- Stefano Pieraccini (Dept of Chemistry, University of Milan, Italy)
- Sara Pellegrino (Dept of Pharmaceutical Sciences, University of Milan, Italy)
- Silvia Gazzola (Dept of Science and High technology, University of Insubria, Italy)
- Oscar Burrone (International Centre for Genetic Engineering and Biotechnology, Trieste, Italy)
- Alberto Barbiroli (Dept of Food, Environmental and Nutritional Sciences, University of Milan, Italy)

Financial support:

- SAVIOR Project: Selecting AntiViral Inhibitors Of Rotavirus.
- FIRB-Futuro in Ricerca 2013-2016. Grant number: RBFR1309E (PI: Dr. Michela Bollati)

Key publications:

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion. Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M. Biochem Biophys Res Commun. 2019 Oct 8;518(1):94-99

Identification of a Small Molecule That Compromises the Structural Integrity of Viroplasms and Rotavirus Double-Layered Particles. Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, E, Ackermann M, Burrone OR, Arnoldi F. J Virol. 2018 Jan 17;92(3):e01943-17

Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa. Bollati M, Villa R, Gourlay LJ, Benedet M, Dehò G, Polissi A, Barbiroli A, Martorana AM, Sperandeo P, Bolognesi M, Nardini M. FEBS J. 2015 May;282(10):1980-97

Structures of the lamin A/C R335W and E347K mutants: implications for dilated cardiolaminopathies. Bollati M, Barbiroli A, Favalli V, Arbustini E, Charron P, Bolognesi M. Biochem Biophys Res Commun. 2012 Feb 10;418(2):217-21.

The SARS-unique domain (SUD) of SARS coronavirus contains two macrodomains that bind G-quadruplexes. Tan J, Vonrhein C, Smart OS, Bricogne G, Bollati M, Kusov Y, Hansen G, Mesters JR, Schmidt CL, Hilgenfeld R. PLoS Pathog. 2009 May;5(5):e1000428.

Publications (11):

Impairing protein–protein interactions in an essential tRNA modification complex: An innovative antimicrobial strategy against Pseudomonas aeruginosa
Bollati, M.; Fasola, E.; Pieraccini, S.; Freddi, F.; Cocomazzi, P.; Oliva, F.; Klussmann, M.; Maspero, A.; Piarulli, U.; Ferrara, S.; Pellegrino, S.; Bertoni, G.; Gazzola, S.
JOURNAL OF PEPTIDE SCIENCE, 31, 2025
DOI: 10.1002/psc.3658

Studying an orphan disease by a biophysical approach: The case of cblC
Vilasi, S.; Longo, L.; Randazzo, L.; Bollati, M.; Carrotta, R.; Costa, M. A.; De Rosa, M.; Mangione, M. R.; Martorana, V.; Milani, M.; Ortore, M. G.; Tutone, M.; Passantino, R.
IL NUOVO CIMENTO C, 47, 2024
DOI: 10.1393/ncc/i2024-24318-2

Accurate and Efficient SAXS/SANS Implementation Including Solvation Layer Effects Suitable for Molecular Simulations
Ballabio, Federico; Paissoni, Cristina; Bollati, Michela; de Rosa, Matteo; Capelli, Riccardo; Camilloni, Carlo
JOURNAL OF CHEMICAL THEORY AND COMPUTATION, 19, 2023
DOI: 10.1021/acs.jctc.3c00864

A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation
Bollati, Michela; Diomede, Luisa; Giorgino, Toni; Natale, Carmina; Fagnani, Elisa; Boniardi, Irene; Barbiroli, Alberto; Alemani, Rebecca; Beeg, Marten; Gobbi, Marco; Fakin, Ana; Mastrangelo, Eloise; Milani, Mario; Presciuttini, Gianluca; Gabellieri, Edi; Cioni, Patrizia; de Rosa, Matteo
computational and structural biotechnology journalissn: 2001-0370chalmers university of technology,, 19, 2021
DOI: 10.1016/j.csbj.2021.11.025

Clinical and histopathological features of gelsolin amyloidosis associated with a novel gsn variant p.Glu580lys
Potrc M.; Volk M.; de Rosa M.; Pizem J.; Teran N.; Jaklic H.; Maver A.; Drnovsek-Olup B.; Bollati M.; Vogelnik K.; Hocevar A.; Gornik A.; Pfeifer V.; Peterlin B.; Hawlina M.; Fakin A.
international journal of molecular sciences (print)issn: 1661-6596molecular diversity preservation international mdpi,, 22, 2021
DOI: 10.3390/ijms22031084

The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
Matteo de Rosa, Alberto Barbiroli, Francesco Bonì, Emanuele Scalone, Davide Mattioni, Maria A Vanoni, Marco Patrone, Michela Bollati, Eloise Mastrangelo, Toni Giorgino, Mario Milani
European biophysics journal, 49, 2020
DOI: 10.1007/s00249-019-01409-9

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion
Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M
Biochemical and biophysical research communications, 518, 2019
DOI: 10.1016/j.bbrc.2019.08.013

The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
de Rosa M, Barbiroli A, Bonì F, Scalone E, Mattioni D, Vanoni MA, Patrone M, Bollati M, Mastrangelo E, Giorgino T, Milani M
European biophysics journal, 49, 2019
DOI: 10.1007/s00249-019-01409-9

Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles
Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, Mastrangelo E, Ackermann M, Burrone OR, Arnoldi F
Journal of virology, 92, 2018
DOI: 10.1128/JVI.01943-17

Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses.
Mastrangelo, E; Bollati, M; Milani, M; De Lamballerie, X; Brisbarre, N; Dalle, K; Lantez, V; Egloff, MP; Coutard, B; Canard, B; Gould, E; Forrester, N; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 63, 2006

Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
Mastrangelo, E; Bollati, M; Milani, M; Brisbarre, N; de Lamballerie, X; Coutard, B; Canard, B; Khromykh, A; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 62, 2006