Gabriella Viero

Research staff

GV has a MD in Biomolecular Sciences (Univ. of Padua – Italy) in 1999 and a PhD in Biotechnologies Applied to Biomedical Sciences (Univ. of Verona – Italy) in 2006. GV is Principal Investigator at IBF (Trento) where she is head of a Laboratory interested in studying translational defects of gene expression. GV published 51 papers in peer-reviewed journals (Nat. Cell. Biol.; J. Cell Biol.; Cell Reports; Immunity, eLife, J.Cell Biology; Mol. Cell; Nucleic Acids Research; Sci. Advanced; PNAS; JBC; Bioinformatics and others). GV has been/is coordinator of 4 grants (1 national 3 international) and Scientist in charge of >6 additional grants from national and international funding bodies. Her h-index is currently 22.

orcid://0000-0002-6755-285X,

Contact:

- Email gabriella.viero@ibf.cnr.it
- Phone +39-0461-314033
- Location Trento

Website:

none

Research interests:

- Translational defects in motor neuron diseases (Principal investigator)
- Integrated approaches to study translational defects in neurodegenerative diseases and bacterial infections (Principal investigator)
- Towards deciphering ribosome heterogeneity and polyribosome code to understand translational control of gene expression (Principal investigator)

Further collaborations:

- Thomas Gillingwater / SMA/ Anatomy and Motor Neuron Diseases/University of Edinburgh (UK)
- Ludo van der Pool and Ewout Groen/ SMA and ALS/ UMC Utrecht Brain Center (NL)
- Giangiacomo Consalez / Neuropathology/ San Raffaele Institute Milan/ (I)
- Pietro Fratta and Giampietro Schiavo / ALS and axonal transport/ UCL (UK)
- Peter Claus/ SMA/ Hannover Medical School (G)
- Toma Tebaldi / RNA data Science/ Yale University University of Trento (US/I)
- Stephanie Halene / RNA Biology and cancer/ Yale University (US)
- Gregor Anderluh and Marjetka Podobnik/ Structural biophysics and protein membrane interaction/ NIC (SLO)
- Marina Boido / SMA and ALS/ University of Turin (I)
- Lorenzo Lunelli / Atomic Force Microscopy/ Bruno Kessler Foundation (I)
- Alessandro Quattrone / Translational genomics/ University of Trento (I)
- Massimiliano Clamer/ CEO Immagina Biotech/ Trento (I)
- Robert Gilbert /Strubi/ University of Oxford (UK)
- Sander Granneman / RNA biology/University of Edinburgh (UK)
- Lorenzo Lunelli / Atomic Force Microscopy/ Bruno Kessler Foundation (I)
- Andrea Passerini and Enrico Blanzieri/ Machine learning and AI/ University of Trento (I)
- Guido Sanguinetti / Statistics and computational biology/ SISSA Trieste (I)
- Claudio Moser / Plant genomics / Edmund Mach Foundation/ Trento (I)

Financial support:

- Axonal translatome in mouse models of Amyotrophic Lateral Scleorosis. 2018-2021 ARISLA (I): Full Grant - Scientist in charge.
- Tagfree axonal translatome to reveal defects in mRNA transport and association to axonal polysomes in ALS/FTD. 2017-2018 ALS Association (USA): Starter Grant - Principal Investigator.

Key publications:

Lauria F, Bernabò P, Tebaldi T, Groen EJN, Perenthaler E, Maniscalco F, Rossi A, Donzel D, Clamer M, Marchioretto M, Omersa N, Orri J, Dalla Serra M, Anderluh G, Quattrone A, Inga A, Gillingwater TH, Viero G.SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol. 2020 Oct;22(10):1239-1251.

Clamer M, Tebaldi T, Lauria F, Bernabò P, Gómez-Biagi RF, Marchioretto M, Kandala DT, Minati L, Perenthaler E, Gubert D, Pasquardini L, Guella G, Groen EJN, Gillingwater TH, Quattrone A, Viero G Active ribosome profiling with RiboLace 2018 Cell Rep. 2018 Oct 23;25(4):1097-1108.e5.

Lauria F, Tebaldi T, Bernabò P, Groen EJN, Gillingwater TH, Viero G. riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data. PLoS Comput Biol. 2018 Aug 13;14(8):e1006169.

Lauria F, Tebaldi T, Lunelli L, Struffi P, Gatto P, Pugliese A, Brigotti M, Montanaro L, Ciribilli Y, Inga A, Quattrone A, Sanguinetti G, Viero G. RiboAbacus: a model trained on polyribosome images predicts ribosome density and translational efficiency from mammalian transcriptomes. Nucleic Acids Res. 2015 Dec 15;43(22):e153.

Viero G, Lunelli L, Passerini A, Bianchini P, Gilbert RJ, Bernabò P, Tebaldi T, Diaspro A, Pederzolli C, Quattrone A*. Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes. J Cell Biol. 2015 Mar 2;208(5):581-96.

Publications (33):

The matricellular protein Connective Tissue Growth Factor promotes motor axon regeneration
Negro S.; Lauria F.; Stazi M.; Tebaldi T.; D'Este G.; Pirazzini M.; Megighian A.; Lessi F.; Mazzanti C. M.; Sales G.; Romualdi C.; Viero G. and Rigoni M.
GLIA (N.Y.N.Y. : Print), 2021

Temporal and tissue-specific variability of SMN protein levels in mouse models of spinal muscular atrophy
Groen EJN, Perenthaler E, Courtney NL, Jordan CY, Shorrock HK, van der Hoorn D, Huang YT, Murray LM, Viero G, Gillingwater TH
Human molecular genetics (Print), 2018
DOI: 10.1093/hmg/ddy195

riboWaltz: Optimization of ribosome P-site positioning in ribosome profiling data
Lauria F, Tebaldi T, Bernabò P, Groen E, Gillingwater T, Viero G
PLOS computational biology (Online), 2018
DOI: 10.1371/journal.pcbi.1006169

In Vivo Translatome Profiling in Spinal Muscular Atrophy Reveals a Role for SMN Protein in Ribosome Biology
Bernabò P, Tebaldi T, Groen EJN, Lane FM, Perenthaler E, Mattedi F, Newbery HJ, Zhou H, Zuccotti P, Potrich V, Shorrock HK, Muntoni F, Quattrone A, Gillingwater TH, Viero G
Cell reports, 2017
DOI: 10.1016/j.celrep.2017.10.010

The Typhoid Toxin Promotes Host Survival and the Establishment of a Persistent Asymptomatic Infection
Del Bel Belluz L, Guidi R, Pateras IS, Levi L, Mihaljevic B, Rouf SF, Wrande M, Candela M, Turroni S, Nastasi C, Consolandi C, Peano C, Tebaldi T, Viero G, Gorgoulis VG, Krejsgaard T, Rhen M, Frisan T
PLOS pathogens, 2016
DOI: 10.1371/journal.ppat.1005528

RiboAbacus: a model trained on polyribosome images predicts ribosome density and translational efficiency from mammalian transcriptomes
Lauria, Fabio; Tebaldi, Toma; Lunelli, Lorenzo; Struffi, Paolo; Gatto, Pamela; Pugliese, Andrea; Brigotti, Maurizio; Montanaro, Lorenzo; Ciribilli, Yari; Inga, Alberto; Quattrone, Alessandro; Sanguinetti, Guido; Viero, Gabriella
Nucleic acids research, 2015
DOI: 10.1093/nar/gkv781

Studying translational control in non-model stressed organisms by polysomal profiling
Bernabo P.; Lunelli L.; Quattrone A.; Jousson O.; Lencioni V.; Viero G.
Journal of insect physiology, 2015
DOI: 10.1016/j.jinsphys.2015.03.011

Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes
Viero G.; Lunelli L.; Passerini A.; Bianchini P.; Gilbert R.J.; Bernabo P.; Tebaldi T.; Diaspro A.; Pederzolli C.; Quattrone A.
The journal of cell biology (Online), 2015
DOI: 10.1083/jcb.201406040

Global translation variations in host cells upon attack of lytic and sublytic Staphylococcus aureus alpha-haemolysin.
Clamer, Massimiliano; Tebaldi, Toma; Marchioretto, Marta; Bernabo, Paola; Bertini, Efrem; Guella, Graziano; Dalla Serra, Mauro; Quattrone, Alessandro; Viero, Gabriella
Biochemical journal (Online), 2015
DOI: 10.1042/BJ20150284

eIF6 coordinates insulin sensitivity and lipid metabolism by coupling translation to transcription
Brina D.; Miluzio A.; Ricciardi S.; Clarke K.; Davidsen P.K.; Viero G.; Tebaldi T.; Offenhauser N.; Rozman J.; Rathkolb B.; Neschen S.; Klingenspor M.; Wolf E.; Gailus-Durner V.; Fuchs H.; Hrabe De Angelis M.; Quattrone A.; Falciani F.; Biffo S.
Nature communications, 2015
DOI: 10.1038/ncomms9261

Identification of essential proteins based on ranking Edge-Weights in Protein-Protein Interaction networks
Wang Y.; Sun H.; Du W.; Blanzeri E.; Viero G.; Xu Y.; Liang Y.
PloS one, 2014
DOI: 10.1371/journal.pone.0108716

Detection of 3-end RNA uridylation with a protein nanopore
Clamer M.; Hofler L.; Mikhailova E.; Viero G.; Bayley H.
ACS nano (Online), 2014
DOI: 10.1021/nn4050479

PTRcombiner: Mining combinatorial regulation of gene expression from post-transcriptional interaction maps
Corrado G.; Tebaldi T.; Bertamini G.; Costa F.; Quattrone A.; Viero G.; Passerini A.
BMC genomics, 2014
DOI: 10.1186/1471-2164-15-304

tRanslatome: an R/Bioconductor package to portray translational control
Tebaldi, Toma; Dassi, Erik; Kostoska, Galena; Viero, Gabriella; Quattrone, Alessandro
Bioinformatics (Oxf., Print), 2014
DOI: 10.1093/bioinformatics/btt634

mRNA fate Life and death of the mRNA in the cytoplasm
Denti MA, Viero G, Provenzani A, Quattrone A, Macchi P
RNA biology, 2013
DOI: 10.4161/rna.23770

p-Sulfonato-calix[n]arenes inhibit staphylococcal bicomponent leukotoxins by supramolecular interactions
Laventie BJ1, Potrich C, Atmanène C, Saleh M, Joubert O, Viero G, Bachmeyer C, Antonini V, Mancini I, Cianferani-Sanglier S, Keller D, Colin DA, Bourcier T, Anderluh G, van Dorsselaer A, Dalla Serra M, Prévost G
Biochemical journal (Lond., 1984), 2013
DOI: 10.1042/BJ20121628

Membrane Damage by an alpha-Helical Pore Forming Protein Equinatoxin II Proceeds Through Succesion of Ordered Steps
Nejc Rojko1, Katarina ?. Kristan1, Gabriella Viero2, Eva ?erovnik3, Peter Ma?ek1, Mauro Dalla Serra2, Gregor Anderluh1,4,5
The Journal of biological chemistry (Print), 2013
DOI: 10.1074/jbc.M113.481572

y-Hemolysin oligomeric structure and effect of its formation on supported lipid bilayers: an AFM investigation
Alessandrini A; Viero G; Dalla Serra M; Prévost G; Facci P
Biochimica et biophysica acta. Biomembranes, 2013
DOI: 10.1016/j.bbamem.2012.09.027

Downregulation of HuR as a new mechanism of doxorubicin resistance in breast cancer cells
Latorre E; Tebaldi T; Viero G; Spartà AM; Quattrone A; Provenzani A
Molecular cancer, 2012
DOI: 10.1186/1476-4598-11-13

Widespread uncoupling between transcriptome and translatome variations after a stimulus in mammalian cells
Tebaldi T; Re A; Viero G; Pegoretti I; Passerini A; Blanzieri E; Quattrone A
BMC genomics, 2012
DOI: 10.1186/1471-2164-13-220

Human perforin employs different avenues to damage membranes
Praper T, Sonnen A, Viero G, Kladnik A, Froelich CJ, Anderluh G, Dalla Serra M, Gilbert R
Journal of biological chemistry (Online), 2011
DOI: 10.1074/jbc.M110.169417

The Role of miR-103 and miR-107 in Regulation of CDK5R1 Expression and in Cellular Migration
Moncini S, Salvi A, Zuccotti P, Viero G, Quattrone A, Barlati S, De Petro G, Venturin M, Riva P
PloS one, 2011

Perforin activity at membranes leads to invaginations and vesicle formation
Praper T, Sonnen AF, Kladnik A, Andrighetti AO, Viero G, Morris KJ, Volpi E, Lunelli L, Dalla Serra M, Froelich CJ, Gilbert RJ, Anderluh G
Proceedings of the National Academy of Sciences of the United States of America, 2011
DOI: 10.1073/pnas.1107473108

Molecular mechanism of pore formation by actinoporins
Kristan,K., G.Viero, M.Dalla Serra, P.Macek, and G.Anderluh
Toxicon (Oxf.), 2009
DOI: 10.1016/j.toxicon.2009.02.026

A molecular pin to study the dynamics of b-barrel formation in Pore Forming Toxins on erythrocytes: a sliding model
G. Viero;a* A. Gropuzzo;a O. Joubert;b+ D. Keller;b G. Prevost;b and M. Dalla Serra;a
Cellular and molecular life sciences (Print. ed.), 2008
DOI: 10.1007/s00018-007-7491-2

Interaction with model membranes and pore formation by human stefin B; studying the native and prefibrillar states.
Rabzelj S., Viero G., Gutierrez-Aguirre I., Turk V., Dalla Serra M., Anderluh G., and Zerovnik E.
The FEBS journal (Print), 2008
DOI: 10.1111/j.1742-4658.2008.06390.x

The Equinatoxin N-Terminus is Transferred Across Planar Bilayers and Helps to Stabilise the Transmembrane Pore
Kristan K., Viero G., Maèek P., Dalla Serra M., Anderluh, G.
The FEBS journal (Print), 2007
DOI: 10.1111/j.1742-4658.2006.05608.x

Engineered covalent leucotoxin heterodimers form functional pores: insights into S-F interactions.
Olivier JOUBERT;* Gabriella VIERO;+ Daniel KELLER;* Eric MARTINEZ;* Didier A. COLIN;* Henri MONTEIL;* Lionel MOUREY;? Mauro DALLA SERRA;+ and Gilles PREVOST;*1
Biochemical journal (Lond., 1984), 2006
DOI: 10.1042/BJ20051878

Homologous versus heterologous interactions in the pore of bicomponent staphylococcal g -hemolysins.
Gabriella VIERO;*2 Romina CUNACCIA;*2 Gilles PREVOST;+ Sandra WERNER;+3 Henri MONTEIL;+ Daniel KELLER;+ Olivier JOUBERT;+ Gianfranco MENESTRINA;* and Mauro DALLA SERRA;*4
Biochemical journal (Lond., 1984), 2006
DOI: 10.1042/BJ20051210

Staphylococcus aureus bi-component g-hemolysins, HlgA, HlgB and HlgC can form mixed pores containing all components.
Mauro Dalla Serra;*+ Manuela Coraiola;+ Gabriella Viero;+ Massimiliano Comai;+ Cristina Potrich;+ Mercedes Ferreras;+| Lamine Baba-Moussa;?^ Didier A. Colin;? Gianfranco Menestrina;+ Sucharit Bhakdi;§ and Gilles Prevost;?
Journal of chemical information and modeling, 2005
DOI: 10.1021/ci050175y

Ion channels and bacterial infection: the case of beta-barrel pore-forming protein toxins of Staphylococcus aureus.
Menestrina G., Dalla Serra M., Comai M., Coraiola M., Viero G., Werner S., Colin D.A., Monteil H., Prévost G.
FEBS letters (Print), 2003
DOI: 10.1016/S0014-5793(03)00850-0

A novel mechanism of pore formation - Membrane penetration by the N-terminal amphipathic region of equinatoxin
Petra Malovrh, Gabriella Viero, Mauro Dalla Serra, Zdravko Podlesek, Jeremy H. Lakey, Peter Macek, Gianfranco Menestrina, and Gregor Anderluh
The Journal of biological chemistry (Print), 2003
DOI: 10.1074/jbc.M300622200

Pore formation by equinatoxin II, a eukaryotic protein toxin, occurs by induction of nonlamellar lipid structures
Gregor Anderluh?, Mauro Dalla Serra§, Gabriella Viero§, Graziano Guella¶, Peter Macek?, and Gianfranco Menestrina§
The Journal of biological chemistry (Print), 2003
DOI: 10.1074/jbc.M305916200