Matteo De Rosa

Research staff

2016-ongoing Researcher, Institute of Biophysics IBF-CNR
2014-2015 Postdoc, Dep. of Biosciences, University of Milano, Italy
2010-2013 Postdoc, IGC, Oeiras, Portugal
2008-2009 Postdoc, ITQB, University of Lisbon, Portugal
2004-2007 PhD in Molecular and Cell Biology, University of Milano, Italy 2004
MSc in Biological Sciences (biomolecular program)

orcid://0000-0002-8634-5426,

Contact:

- Email matteo.derosa@cnr.it
- Phone +39-02-23699548
- Location Milano

Research interests:

- Gelsolin physiopathology (Principal investigator)
- Widespread occurrence and novel functions of proteins binding Z-DNA/RNA, the left-handed helix of nucleic acids (Principal investigator)
- Structure-based drug discovery on viral proteins
- Structural analysis of proteins involved in various human diseases and research of new therapeutic strategies
- Applications of high-throughput molecular dynamics methods
- Structure-based drug discovery

Further collaborations:

- Luisa Diomede (Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy)
- Marco Gobbi (Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy)
- Marten Beeg (Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy)
- Alberto Barbiroli (Università di Milano, Milano, Italy)
- Vittoria Brambilla (Università di Milano, Milano, Italy)
- Maria Antonietta Vanoni (Università di Milano, Milano, Italy)
- Sara Pellegrino (Università di Milano, Milano, Italy)
- Carlo Camilloni (Università di Milano, Milano, Italy)
- Alessandro Aliverti (Università di Milano, Milano, Italy)
- Ana Fakin (Ljubljana University Medical Centre, Ljubljana, Slovenia)
- Jan Gettemans (Ghent University, Ghent, Belgium)
- Angelica Crottini (CIBIO, Porto, Portugal)

Financial support:

- 2023-2025 - Progetti di Rilevante Interesse Nazionale (PRIN 2022) “NAGAT: Novel Approaches to Gelsolin Amyloidosis Treatment” (ref 20224JEF9E) finanziato come Principal Investigator (PI).
- 2023-2025 - Cariplo-Telethon Joint Grant “Broad-spectrum rescue-of-secretion of Tdark glycoprotein mutants” (ref GJC22077), ruolo team member (PI Dr. Pietro Roversi)
- 2023 – Telethon seed grant “Role of endoplasmic reticulum in cells carrying R257C ACTG2 mutant” (Ref. GSA22Q001), ruolo: collaborator (PI: Dr. Federica Viti)
- 2023 - MOSBRI transnational access “Characterization of conformational fluctuations of a flexible multi-domain protein: the case of Gelsolin” (ref MOSBRI-2023-168) finanziamento per accesso ad infrastrutture di ricerca, ruolo PI
- 2022-2025 - Partenariati estesi alle università, ai centri di ricerca, alle aziende per il finanziamento di progetti di ricerca di base” finanziato dal NextGenerationEU “One Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases” (ref: PE00000007) ruolo: membro del team (PI Dr. Eloise Mastrangelo).
- 2022-2024 - Rafforzamento e creazione di Infrastrutture di Ricerca da finanziare nell’ambito del PNRR “EBRAINS-Italy - European Brain ReseArch INfrastructureS-Italy” (ref: IR0000011) ruolo: membro del team (PI Dr. Eloise Mastrangelo).
- 2022 - RESEARCH GRANTS HCU Network America and CblC onlus “Identification of compounds to rescue MMACHC functional deficiency in cblC disease", ruolo: team member (PI: Dr. Silvia Vilasi)
- 2019 - iNEXT 'Impact of pathological mutations on gelsolin structure and dynamics' (PID: 8038), finanziamento per accesso ad infrastrutture di ricerca, ruolo PI
- 2018-2019 - Amyloidosis Foundation research grant “From protein structure to novel drugs against Agel amyloidosis: a fast and cost-effective route to the development of new therapeutics”, finanziato come PI
- 2016-2017 - Telethon exploratory grant “Characterization of recently identified gelsolin variants responsible for a novel renal amyloidosis and in silico screening of drug candidates” (ref GEP15070) finanziato come PI
- 2011-2013 - FCT Post Doctoral Fellowship (ref SFRH/BPD/71629/2010)
- 2010 - Premio SIB in memoria di Prof. Bruno Curti
- 2009 - FEBS short term fellowship

Key publications:

Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant. Giorgino T, Mattioni D, Hassan A, Milani M, Mastrangelo E, Barbiroli A, Verhelle A, Gettemans J, Barzago MM, Diomede L, de Rosa M. Biochim Biophys Acta Mol Basis Dis. 2019 Mar 1;1865(3):648-660. doi: 10.1016/j.bbadis.2019.01.010. Epub 2019 Jan 6. PMID: 30625383

Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein. Bonì F, Milani M, Barbiroli A, Diomede L, Mastrangelo E, de Rosa M. Hum Mol Genet. 2018 Jan 1;27(1):53-65. doi: 10.1093/hmg/ddx383. PMID: 29069428

The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties. de Rosa M, Barbiroli A, Bonì F, Scalone E, Mattioni D, Vanoni MA, Patrone M, Bollati M, Mastrangelo E, Giorgino T, Milani M. Eur Biophys J. 2020 Jan;49(1):11-19. doi: 10.1007/s00249-019-01409-9. Epub 2019 Nov 13. PMID: 31724080

Conformational dynamics in crystals reveal the molecular bases for D76N beta-2 microglobulin aggregation propensity. Le Marchand T, de Rosa M, Salvi N, Sala BM, Andreas LB, Barbet-Massin E, Sormanni P, Barbiroli A, Porcari R, Sousa Mota C, de Sanctis D, Bolognesi M, Emsley L, Bellotti V, Blackledge M, Camilloni C, Pintacuda G, Ricagno S. Nat Commun. 2018 Apr 25;9(1):1658. doi: 10.1038/s41467-018-04078-y. PMID: 29695721

Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant. Bonì F, Milani M, Porcari R, Barbiroli A, Ricagno S, de Rosa M. Sci Rep. 2016 Sep 16;6:33463. doi: 10.1038/srep33463. PMID: 27633054

Publications (15):

Covalent inhibition of P. falciparum ferredoxin-NADP plus reductase: Exploring alternative strategies for the development of new antimalarial drugs
de Rosa, Matteo and Nonnis, Simona and Aliverti, Alessandro
Biochemical and biophysical research communications (Print), 2021
DOI: 10.1016/j.bbrc.2021.09.008

A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation
Bollati, Michela; Diomede, Luisa; Giorgino, Toni; Natale, Carmina; Fagnani, Elisa; Boniardi, Irene; Barbiroli, Alberto; Alemani, Rebecca; Beeg, Marten; Gobbi, Marco; Fakin, Ana; Mastrangelo, Eloise; Milani, Mario; Presciuttini, Gianluca; Gabellieri, Edi; Cioni, Patrizia; de Rosa, Matteo
Computational and Structural Biotechnology Journal, 2021
DOI: 10.1016/j.csbj.2021.11.025

Clinical and histopathological features of gelsolin amyloidosis associated with a novel gsn variant p.Glu580lys
Potrc M.; Volk M.; de Rosa M.; Pizem J.; Teran N.; Jaklic H.; Maver A.; Drnovsek-Olup B.; Bollati M.; Vogelnik K.; Hocevar A.; Gornik A.; Pfeifer V.; Peterlin B.; Hawlina M.; Fakin A.
International journal of molecular sciences (Print), 2021
DOI: 10.3390/ijms22031084

The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
De Rosa Matteo; Barbiroli Alberto; Bonì Francesco; Scalone Emanuele; Mattioni Davide; Vanoni Maria A.; Patrone Marco; Bollati Michela; Mastrangelo Eloise; Giorgino Toni; Milani Mario
European biophysics journal, 2020
DOI: 10.1007/s00249-019-01409-9

An alternative non-proteolytic mechanism may underlie AGel amyloidosis
Bonì F, Milani M, Mastrangelo E, Babiroli A, Diomede L, de Rosa M
Amyloid (Online), 2019
DOI: 10.1080/13506129.2019.1582510

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion
Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M
Biochemical and biophysical research communications (Print), 2019
DOI: 10.1016/j.bbrc.2019.08.013

Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant
Giorgino T, Mattioni D, Hassan A, Milani M, Mastrangelo E, Barbiroli A, Verhelle A, Gettemans J, Barzago MM, Diomede L, de Rosa M
Biochimica et biophysica acta. Molecular basis of disease, 2019
DOI: 10.1016/j.bbadis.2019.01.010

Conformational dynamics in crystals reveal the molecular bases for D76N beta-2 microglobulin aggregation propensity
Le Marchand T.; De Rosa M.; Salvi N.; Sala B.M.; Andreas L.B.; Barbet-Massin E.; Sormanni P.; Barbiroli A.; Porcari R.; Sousa Mota C.; De Sanctis D.; Bolognesi M.; Emsley L.; Bellotti V.; Blackledge M.; Camilloni C.; Pintacuda G.; Ricagno S.
Nature communications, 2018
DOI: 10.1038/s41467-018-04078-y

Structure-based design and molecular profiling of Smac-mimetics selective for cellular IAPs
Corti A, Milani M, Lecis D, Seneci P, de Rosa M, Mastrangelo E, Cossu F
The FEBS journal (Print), 2018
DOI: 10.1111/febs.14616

Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles
Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, Mastrangelo E, Ackermann M, Burrone OR, Arnoldi F
Journal of virology (Print), 2018
DOI: 10.1128/JVI.01943-17

Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein
Bonì F, Milani M, Barbiroli A, Diomede L, Mastrangelo E, de Rosa M
Human molecular genetics online, 2018
DOI: 10.1093/hmg/ddx383

Antagonistic Transcription Factor Complexes Modulate the Floral Transition in Rice
Brambilla V, Martignago D, Goretti D, Cerise M, Somssich M, de Rosa M, Galbiati F, Shrestha R, Lazzaro F, Simon R, Fornara F
The Plant cell, 2017
DOI: 10.1105/tpc.17.00645

Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant
Bonì F, Milani M, Porcari R, Barbiroli A, Ricagno S, de Rosa M
Scientific reports (Nature Publishing Group), 2016
DOI: 10.1038/srep33463.

Rational design of mutations that change the aggregation rate of a protein while maintaining its native structure and stability
Camilloni C, Sala BM, Sormanni P, Porcari R, Corazza A, De Rosa M, Zanini S, Barbiroli A, Esposito G, Bolognesi M, Bellotti V, Vendruscolo M, Ricagno S
Scientific reports (Nature Publishing Group), 2016
DOI: 10.1038/srep25559

Decoding the Structural Bases of D76N beta 2-Microglobulin High Amyloidogenicity through Crystallography and Asn-Scan Mutagenesis
de Rosa, Matteo; Barbiroli, Alberto; Giorgetti, Sofia; Mangione, Patrizia P.; Bolognesi, Martino; Ricagno, Stefano
PloS one, 2015
DOI: 10.1371/journal.pone.0144061