Laura Lagostena

Research staff

Education / Training:
01/1999 - 04/2002: PhD in Neuroscience “Calcium Dynamics in Sensory and Supporting Cells of the Organ of Corti”, Open University, London, United Kingdom
09/1984 - 05/1991Degree in biology “Neurotoxic effect of a phospholipase isolated from snake venom on ion currents in cultured neurons”, University of Genova (Italy)

Job Positions:
12/2018 - Present: Researcher (permanent position) at Istituto di Biofisica - CNR
10/2016 - 12/2018: Post Doc at Fondazione Telethon - Istituto di Biofisica
09/2013 - 09/2016: Research Fellow at Istituto di Biofisica - CNR
09/2012 - 08/2013: Post Doc at Istituto di Tecnologia (IIT)
12/2008 - 12/2010: Research Fellow at Dipartimento di Scienze Biomediche - Università di Modena
10/2005 - 12/2008: Research Fellow at Settore di Neurobiologia - SISSA
05/2003 - 12/2004: Post Doc at Istituto Dermopatico dell'Immacolata (IDI)
10/1998 - 05/2003: Research Fellow at Scuola Internazionale degli Studi Superiori Applicati (SISSA)
12/1995 - 08/1998: Scholarship at Istituto di Cibernetica e Biofisica - CNR
06/1991 - 07/1994: Scholarship at Ospedale Civile di Sondrio

orcid://0000-0001-6440-3720,

Contact:

- Email laura.lagostena@ibf.cnr.it
- Phone +39-010-6475560
- Location Genova

Research interests:

- Neurotoxic effect of per- and polyfluoroalkyl substances (PFAS) (Principal investigator)
- Sensory organs (hearing and olfaction) (Principal investigator)
- Synaptic transmission and plasticity (Principal investigator)
- Functional and structural studies of human CLC protein complexes involved in genetic diseases
- Recordings of electronic currents from plant cytochromes

Key publications:

Di Zanni E, Palagano E, Lagostena L, Strina D, Rehman A, Abinun M, De Somer L, Martire B, Brown J, Kariminejad A, Balasubramaniam S, Baynam G, Gurrieri F, Pisanti MA, De Maggio I, Abboud MR, Chiesa R, Burren CP, Villa A, Sobacchi C, Picollo A, . "Pathobiologic Mechanisms of Neurodegeneration in Osteopetrosis Derived From Structural and Functional Analysis of 14 ClC-7 Mutants". Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research. 2021 36(3):531-545

Badurek S, Griguoli M, Asif-Malik A, Zonta B, Guo F, Middei S, Lagostena L, Jurado-Parras MT, Gillingwater TH, Gruart A, Delgado-García JM, Cherubini E, Minichiello L, "Immature Dentate granule Cells Require Ntrk2/Trkb for the Formation of Functional Hippocampal Circuitry". iScience. 2020 23 (5).

Lagostena L, Zifarelli G, Picollo A, "New Insights into the Mechanism of NO3 - Selectivity in the Human Kidney Chloride Channel ClC-Ka and the CLC Protein Family". Journal of the American Society of Nephrology : JASN. 2019 30 (2).

Lagostena L, Rosato-Siri M, D'Onofrio M, Brandi R, Arisi I, Capsoni S, Franzot J, Cattaneo A, Cherubini E, "In the adult hippocampus, chronic nerve growth factor deprivation shifts GABAergic signaling from the hyperpolarizing to the depolarizing direction". The Journal of neuroscience : the official journal of the Society for Neuroscience. 2010 30 (3).

Lagostena L, Danober L, Challal S, Lestage P, Mocaër E, Trocmé-Thibierge C, Cherubini E, "Modulatory effects of S 38232, a non alpha-7 containing nicotine acetylcholine receptor agonist on network activity in the mouse hippocampus". NEUROPHARMACOLOGY. 2010 58 (4-5).

Publications (36):

Functional characterisation of electron currents in plant vacuoles: direct recordings of cytochrome b561A activity
Carpaneto Armando; Gradogna Antonella; Lagostena Laura; Beltrami Sara; Tosato Edoardo; Picco Cristiana; Scholz-Starke Joachim; Sparla Francesca; Trost Paolo
European biophysics journal, 2023

Tonoplast cytochrome b561 is a transmembrane ascorbate-dependent monodehydroascorbate reductase: functional characterization of electron currents in plant vacuoles
Gradogna, Antonella; Lagostena, Laura; Beltrami, Sara; Tosato, Edoardo; Picco, Cristiana; Scholz-Starke, Joachim; Sparla, Francesca; Trost, Paolo; Carpaneto, Armando
New phytologist (Print), 2023
DOI: 10.1111/nph.18823

Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters
Festa Margherita; Minicozzi Velia; Boccaccio Anna; Lagostena Laura; Gradogna Antonella; Qi Tianwen; Costa Alex; Larisch Nina; Hamamoto Shin; *Pedrazzini Emanuela; Milenkovic Stefan; Scholz-Starke Joachim; Ceccarelli Matteo; *Vitale Alessandro; Dietrich Petra; Uozumi Nobuyuki; Gambale Franco; Carpaneto Armando.
Cells, 2022
DOI: 10.3390/cells11060921

Pathobiologic Mechanisms of Neurodegeneration in Osteopetrosis Derived from Structural and Functional Analysis of 14 ClC-7 Mutants
Eleonora Di Zanni, Eleonora Palagano, Laura Lagostena, Dario Strina , Asma Rehman, Mario Abinun, Lien De Somer, Baldassarre Martire, Justin Brown, Ariana Kariminejad, Shanti Balasubramanian, Gareth Baynam, Fiorella Gurrieri, Maria A Pisanti, Ilaria De Maggio, Miguel R Abboud, Robert Chiesa, Christine P Burren, Anna Villa, Cristina Sobacchi, Alessandra Picollo
Journal of bone and mineral research, 2021
DOI: 10.1002/jbmr.4200

The mechanism and energetics of a ligand-controlled hydrophobic gate in a mammalian two pore channel
Milenkovic S.; Bodrenko I.V.; Lagostena L.; Gradogna A.; Serra G.; Bosin A.; Carpaneto A.; Ceccarelli M.
PCCP. Physical chemistry chemical physics (Print), 2020
DOI: 10.1039/d0cp00805b

Immature Dentate Granule Cells Require Ntrk2/Trkb for the Formation of Functional Hippocampal Circuitry
Badurek S.; Griguoli M.; Asif-Malik A.; Zonta B.; Guo F.; Middei S.; Lagostena L.; Jurado-Parras M.T.; Gillingwater T.H.; Gruart A.; Delgado-Garcia J.M.; Cherubini E.; Minichiello L.
I, science (Print), 2020
DOI: 10.1016/j.isci.2020.101078

New Insights into the Mechanism of NO3 - Selectivity in the Human Kidney Chloride Channel ClC-Ka and the CLC Protein Family
Lagostena L, Zifarelli G, Picollo A
Journal of the American Society of Nephrology, 2019
DOI: 10.1681/ASN.2018060593

A perspective on the modulation of plant and animal two pore channels (TPCs) by the flavonoid naringenin
Benkerrou D.; Minicozzi V.; Gradogna A.; Milenkovic S.; Bodrenko I. V.; Festa M.; Lagostena L.; Cornara L.; D'Amore A.; Ceccarelli M.; Filippini A.; Carpaneto A.
Biophysical chemistry (Print), 2019
DOI: 10.1016/j.bpc.2019.106246

The human two-pore channel 1 is modulated by cytosolic and luminal calcium
Lagostena L, Festa M, Pusch M, Carpaneto A
Scientific reports (Nature Publishing Group), 2017
DOI: 10.1038/srep43900

The signaling lipid phosphatidylinositol-3,5-bisphosphate targets plant CLC-a anion/H exchange activity.
Carpaneto A, Boccaccio A, Lagostena L, Di Zanni E, Scholz-Starke J
EMBO reports (Print), 2017
DOI: 10.15252/embr.201643814

Electron current recordings in living cells
Trost P, Picco C, Scholz-Starke J, Festa M, Lagostena L, Costa A, Sparla F, Carpaneto A
Biophysical chemistry (Print), 2017
DOI: 10.1016/j.bpc.2017.05.006

Naringenin Impairs Two-Pore Channel 2 Activity And Inhibits VEGF-Induced Angiogenesis
Pafumi I, Festa M, Papacci F, Lagostena L, Giunta C, Gutla V, Cornara L, Favia A, Palombi F, Gambale F, Filippini A, Carpaneto A
Scientific reports (Nature Publishing Group), 2017
DOI: 10.1038/s41598-017-04974-1

Identification and Functional Characterization of CLCN1 Mutations Found in Nondystrophic Myotonia Patients
Vindas-Smith R.; Fiore M.; Vasquez M.; Cuenca P.; del Valle G.; Lagostena L.; Gaitan-Penas H.; Estevez R.; Pusch M.; Morales F.
Human mutation, 2016
DOI: 10.1002/humu.22916

Using the plant vacuole as a biological system to investigate the functional properties of exogenous channels and transporters
M. Festa , L. Lagostena , A. Carpaneto
Biochimica et biophysica acta. Biomembranes, 2016
DOI: 10.1016/j.bbamem.2015.09.022

Selective activation of parvalbumin- or somatostatin-expressing interneurons triggers epileptic seizurelike activity in mouse medial entorhinal cortex
Yekhlef, Latefa; Breschi, Gian Luca; Lagostena, Laura; Russo, Giovanni; Taverna, Stefano
Journal of neurophysiology, 2015
DOI: 10.1152/jn.00841.2014

GlialCAM, a CLC-2 Cl- Channel Subunit, Activates the Slow Gate of CLC Chloride Channels
Jeworutzki, Elena; Lagostena, Laura; Elorza-Vidal, Xabier; Lopez-Hernandez, Tania; Estevez, Raul; Pusch, Michael
Biophysical journal (Print), 2014
DOI: 10.1016/j.bpj.2014.07.040

The depolarizing action of GABA controls early network activity in the developing hippocampus
Cherubini, Enrico; Griguoli, Marilena; Safiulina, Victoria; Lagostena, Laura
Molecular neurobiology, 2011
DOI: 10.1007/s12035-010-8147-z

Modulatory effects of S 38232, a non ?-7 containing nicotine acetylcholine receptor agonist on network activity in the mouse hippocampus
Lagostena, Laura; Danober, Laurence; Challal, Sylvie; Lestage, Pierre; Mocaër, Elisabeth; Trocmé-Thibierge, Caryn; Cherubini, Enrico
Neuropharmacology, 2010
DOI: 10.1016/j.neuropharm.2009.12.003

In the adult hippocampus, chronic nerve growth factor deprivation shifts GABAergic signaling from the hyperpolarizing to the depolarizing direction
Lagostena, Laura; Rosato-Siri, Marcelo; D'Onofrio, Mara; Brandi, Rossella; Arisi, Ivan; Capsoni, Simona; Franzot, Jessica; Cattaneo, Antonino; Cherubini, Enrico
The Journal of neuroscience, 2010
DOI: 10.1523/JNEUROSCI.3326-09.2010

The partial ?7 nicotine acetylcholine receptor agonist S 24795 enhances long-term potentiation at CA3-CA1 synapses in the adult mouse hippocampus
Lagostena, Laura; Trocme-Thibierge, Caryn; Morain, Philippe; Cherubini, Enrico
Neuropharmacology, 2008
DOI: 10.1016/j.neuropharm.2007.11.016

Fast adaptation in mouse olfactory sensory neurons does not require the activity of phosphodiesterase.
Boccaccio Anna 1; Lagostena Laura 1; Hagen Volker 2; Menini Anna 1
The Journal of general physiology, 2006
DOI: 10.1085/jgp.200609555

Carbon nanotube substrates boost neuronal electrical signaling
Lovat, Viviana; Pantarotto, Davide; Lagostena, Laura; Cacciari, Barbara; Grandolfo, Micaela; Righi, Massimo; Spalluto, Giampiero; Prato, Maurizio; Ballerini, Laura
Nano letters (Print), 2005
DOI: 10.1021/nl050637m

Electrophysiological properties of mouse bone marrow c-kit⁺ cells co-cultured onto neonatal cardiac myocytes
Lagostena, Laura; Avitabile, Daniele; De Falco, Elena; Orlandi, Alessia; Grassi, Francesca; Iachininoto, Maria Grazia; Ragone, Gianluca; Fucile, Sergio; Pompilio, Giulio; Eusebi, Fabrizio; Pesce, Maurizio; Capogrossi, Maurizio C.
Cardiovascular research, 2005
DOI: 10.1016/j.cardiores.2005.01.018

Olfaction: from odorant molecules to the olfactory cortex.
Menini, Anna; Lagostena, Laura; Boccaccio, Anna
News in physiological sciences, 2004
DOI: 10.1152/nips.1507.2003

Whole-cell recordings and photolysis of caged compounds in olfactory sensory neurons isolated from the mouse
Lagostena, Laura; Menini, Anna
Chemical senses, 2003
DOI: 10.1093/chemse/bjg063

Purinergic control of intercellular communication between Hensen's cells of the guinea-pig cochlea
Lagostena, L; Ashmore, JF; Kachar, B; Mammano, F
Journal of physiology (Lond., Print), 2001
DOI: 10.1111/j.1469-7793.2001.0693h.x

Intracellular calcium dynamics and membrane conductance changes evoked by Deiters' cell purinoceptor activation in the organ of Corti
Lagostena, T; Mammano, F
Cell calcium (Edinburgh), 2001
DOI: 10.1054/ceca.2000.0183

Frequency dependence of electrical coupling in Deiters' cells of the guinea pig cochlea
Lagostena, L; Cicuttin, A; Inda, J; Kachar, B; Mammano, F
Cell adhesion and communication (Softback), 2001
DOI: 10.3109/15419060109080760

Cholinergic control of membrane conductance and intracellular free Ca2+ in outer hair cells of the guinea pig cochlea
Evans, MG; Lagostena, L; Darbon, P; Mammano, F
Cell calcium (Edinburgh), 2000
DOI: 10.1054/ceca.2000.0145

Frequency dependence of electrical coupling in deiters' cells of the guinea pig cochlea
Lagostena, Laura; Cicuttin, Andres; Inda, Juan; Kachar, Bechara; Mammano, Fabio
Cell adhesion and communication (Softback), 2000

Large-conductance calcium-activated anion channel characteristics in neuroblastoma cells
Nobile, M.; Lagostena, L.
General physiology and biophysics (Tlacené vyd.), 2000

ATP-induced Ca2+ release in cochlear outer hair cells: Localization of an inositol triphosphate-gated Ca2+ store to the base of the sensory hair bundle
Mammano, F; Frolenkov, GI; Lagostena, L; Belyantseva, IA; Kurc, M; Dodane, V; Colavita, A; Kachar, B
The Journal of neuroscience, 1999

Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system
Colavita, A.; Capello, G.; Ijaduola, R. B.; Cunei, A.; Lagostena, L.; Canepari, M.; Mammano, F.
Proceedings of SPIE, the International Society for Optical Engineering, 1999

A discriminant block among K⁺ channel types by phenytoin in neuroblastoma cells
Nobile, M.; Lagostena, L.
British journal of pharmacology, 1998
DOI: 10.1038/sj.bjp.0702035

Functional aspects of dexamethasone upregulated nicotinic acetylcholine receptors in C2C12 myotubes
Maestrone, E.; Lagostena, L.; Henning, R. H.; Den Hertog, A.; Nobile, Mario
Pharmacological research (Print), 1995
DOI: 10.1016/S1043-6618(05)80019-2

The toxin helothermine affects potassium currents in newborn rat cerebellar granule cells
Nobile, M.; Magnelli, V.; Lagostena, L.; Mochca-Morales, J.; Possani, L. D.; Prestipino, G.
The journal of membrane biology (Print), 1994
DOI: 10.1007/BF00232674