Antonella Gradogna

Research staff

Researcher since 2017.
Post-doc position at IBF-CNR Genoa, Italy (2012-2017).
PhD in “Biosciences and Biotechnology - Biochemistry and Biophysics” at the University of Padua (2012).
Master Degree in Cellular and Molecular Biology at the University of Genoa (2008).
Bachelor’s Degree in Biological Sciences at the University of Genoa (2005).

orcid://0000-0002-4401-9913, ncbi://51088544

Contact:

- Email antonella.gradogna@ibf.cnr.it
- Phone +39-010-6475652
- Location Genova

Website:

https://www.researchgate.net/profile/Antonella_Gradogna

Research interests:

- Functional activity of membrane channels and transporters by electrophysiology and fluorescence. (Principal investigator)
- Recordings of electronic currents from plant cytochromes (Principal investigator)
- Physiology and biophysics of plant vacuolar transport proteins
- Study of the Ca2+-activated chloride channels and phospholipid scramblases of the TMEM16/Anoctamin family
- Electronic transport mediated by proteins belonging to the cytochrome b561 family

Further collaborations:

- Anna Boccaccio per la caratterizzazione di TMEM16E/ANO5.
- Michael Pusch per le misure sui CLC e su VRAC.
- Paolo Trost (FaBiT Università di Bologna)
- Francesca Sparla (FaBiT Università di Bologna)
- Rainer Hedrich (Università di Wuerzburg – Germania)

Key publications:

Di Zanni E*, Gradogna A*, Scholz-Starke J, Boccaccio A. Gain of function of TMEM16E/ANO5 scrambling activity caused by a mutation associated with gnathodiaphyseal dysplasia. Cell Mol Life Sci. 2017. doi: 10.1007/s00018-017-2704-9, *these authors contributed equally.

Gaitán-Peñas H*, Gradogna A*, Laparra-Cuervo L, Solsona C, Fernández-Dueñas V, Barrallo-Gimeno A, Ciruela F, Lakadamyali M, Pusch M, Estévez R. Investigation of LRRC8-mediated volume-regulated anion currents in Xenopus oocytes reveals novel properties. Biophys J. 2016;111(7):1429-1443. doi: 10.1016/j.bpj.2016.08.030, *these authors contributed equally.

Gradogna A, Babini E, Picollo A, Pusch M. A regulatory calcium binding site at the subunit interface of CLC-K kydney chloride channels. J Gen Physiol. 2010;136(3):311-23. doi: 10.1085/jgp.201010455.

Gradogna A, Scholz-Starke J, Gutla PV, Carpaneto A. Fluorescence combined with excised patch: measuring calcium currents in plant cation channels. Plant J. 2009 58:175-82. doi: 10.1111/j.1365-313X.2008.03762.x.

Antonella Gradogna, Joachim Scholz-Starke, José M Pardo, Armando Carpaneto Beyond the patch-clamp resolution:functional activity of non-electrogenic vacuolar nhx proton/potassium antiporters and inhibition by phosphoinositides, New phytologist - 2020, doi: 10.1111/nph.17021

Publications (20):

TMEM16E/ANO5 mutations related to bone dysplasia or muscular dystrophy cause opposite effects on lipid scrambling
Di Zanni Eleonora; Gradogna Antonella; Picco Cristiana; Scholz-Starke Joachim and Boccaccio Anna
Human mutation, 2020
DOI: 10.1002/humu.24006, Early Access Date = MAR 2020

Beyond the patch-clamp resolution:functional activity of non-electrogenic vacuolar NHX proton/potassium antiporters and inhibition by phosphoinositides
Antonella Gradogna, Joachim Scholz-Starke, José M Pardo, Armando Carpaneto
New phytologist (Print), 2020
DOI: 10.1111/nph.17021

The mechanism and energetics of a ligand-controlled hydrophobic gate in a mammalian two pore channel
Milenkovic S.; Bodrenko I.V.; Lagostena L.; Gradogna A.; Serra G.; Bosin A.; Carpaneto A.; Ceccarelli M.
PCCP. Physical chemistry chemical physics (Print), 2020
DOI: 10.1039/d0cp00805b

Lifting the veils on TMEM16E function
Boccaccio A, Di Zanni E, Gradogna A, Scholz-Starke J
Channels (Austin Tex.), 2019
DOI: 10.1080/19336950.2018.1557470

Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa
Böhm J, Messerer M, Müller HM, Scholz-Starke J, Gradogna A, Scherzer S, Maierhofer T, Bazihizina N, Zhang H, Stigloher C, Ache P, Al-Rasheid KAS, Mayer KFX, Shabala S, Carpaneto A, Haberer G, Zhu JK, Hedrich R
Current biology, 2018
DOI: 10.1016/j.cub.2018.08.004

Modulation of calcium and potassium permeation in plant TPC channels
Carpaneto A, Gradogna A
Biophysical chemistry (Print), 2018
DOI: 10.1016/j.bpc.2018.02.006

Gain of function of TMEM16E/ANO5 scrambling activity caused by a mutation associated with gnathodiaphyseal dysplasia
Di Zanni E, Gradogna A, Scholz-Starke J, Boccaccio A
Cellular and molecular life sciences (Print. ed.), 2018
DOI: 10.1007/s00018-017-2704-9

Cisplatin activates volume sensitive LRRC8 channel mediated currents in Xenopus oocytes
Gradogna A.; Gaitan-Penas H.; Boccaccio A.; Estevez R.; Pusch M.
Channels (Austin Tex.), 2017
DOI: 10.1080/19336950.2017.1284717

Subunit-dependent oxidative stress sensitivity of LRRC8 volume-regulated anion channels
Gradogna A, Gavazzo P, Boccaccio A, Pusch M
Journal of physiology (Lond., Print), 2017
DOI: 10.1113/JP274795

Kidney CLC-Kchloride channels inhibitors: structure-based studies and efficacy in hypertension and associated CLC-K polymorphisms
Liantonio A, Imbrici P, Camerino GM, Fracchiolla G, Carbonara G, Giannico D, Gradogna A, Mangiatordi GF, Nicolotti O, Tricarico D, Pusch M, Conte Camerino D
Journal of hypertension, 2016
DOI: 10.1097/HJH.0000000000000876

Investigation of LRRC8-Mediated Volume-Regulated Anion Currents in Xenopus Oocytes
Gaitán-Peñas H, Gradogna A, Laparra-Cuervo L, Solsona C, Fernández-Dueñas V, Barrallo-Gimeno A, Ciruela F, Lakadamyali M, Pusch M, Estévez R
Biophysical journal (Online), 2016
DOI: 10.1016/j.bpj.2016.08.030

Regulatory-auxiliary subunits of CLC chloride channel-transport proteins
Barrallo-Gimeno A.; Gradogna A.; Zanardi I.; Pusch M.; Estevez R.
Journal of physiology (Lond., Print), 2015
DOI: 10.1113/JP270057

Targeting kidney CLC-K channels: Pharmacological profile in a human cell line versus Xenopus oocytes
Imbrici P.; Liantonio A.; Gradogna A.; Pusch M.; Camerino D.C.
Biochimica et biophysica acta. Biomembranes, 2014
DOI: 10.1016/j.bbamem.2014.05.017

I-J loop involvement in the pharmacological profile of CLC-K channels expressed in Xenopus oocytes
Gradogna, Antonella; Imbrici, Paola; Zifarelli, Giovanni; Liantonio, Antpnella; Camerino, Diana Conte; Pusch, Michael
Biochimica et biophysica acta. Biomembranes, 2014
DOI: 10.1016/j.bbamem.2014.07.021

Alkaline pH block of CLC-K kidney chloride channels mediated by a pore lysine residue
Gradogna A, Pusch M
Biophysical journal (Print), 2013
DOI: 10.1016/j.bpj.2013.05.044

Dissecting a regulatory calcium-binding site of CLC-K kidney chloride channels
Gradogna A; Fenollar-Ferrer C; Forrest LR, Pusch M
The Journal of general physiology, 2012
DOI: 10.1085/jgp.201210878

A regulatory calcium-binding site at the subunit interface of CLC-K kidney chloride channels
Gradogna A, Babini E, Picollo A, Pusch M
The Journal of general physiology, 2010
DOI: 10.1085/jgp.201010455

Identification of sites responsible for the potentiating effect of niflumic acid on ClC-Ka kidney chloride channels.
Zifarelli G, Liantonio A, Gradogna A, Picollo A, Gramegna G, De Bellis M, Murgia AR, Babini E, Camerino DC, Pusch M
British journal of pharmacology, 2010
DOI: 10.1111/j.1476-5381.2010.00822.x

Molecular pharmacology of kidney and inner ear CLC-K chloride channels
Gradogna A., Pusch M.

Fluorescence. Combined with excised patch: measuring calcium currents in plant cation channels.
Gradogna A.; Scholz-Starke, J.; Gutla, P. V.; Carpaneto, A.
Plant journal (Print), 2009
DOI: 10.1111/j.1365-313X.2008.03762.x