Rita Carrotta
Research staff
Dal gennaio 2008: Ricercatrice IBF.
2006:2007: Post-Doc presso Dip di Fisica, Università di Palermo
2003-2005: Contratto e assegno di ricerca presso IBF - CNR di Palermo
Maggio 2003: PhD in Biofisica presso KVL, Frederiksberg C, DK
Luglio 1997: Laurea in Fisica, Università di Palermo
Periodi di maternità: Maggio – Ottobre 2009; Gennaio – Luglio 2006
Luglio – Dicembre 2003; Settembre 2001 – Febbraio 2002
Esperienze: spettroscopia, tecniche di diffusione (small angle x-ray and neutron scattering and multi-angle light scattering), calorimetia (DSC and ITC) and microscopia (AFM and light).
orcid://0000-0003-4628-2819,
Contact:
- Email rita.carrotta@ibf.cnr.it
- Phone +39-091-6809313
- Location Palermo
Research interests:
- Amyloid aggregation processes and their inhibition (Principal investigator)
- Model Membrane Systems and Interaction Studies (Principal investigator)
- Liposome as drug delivery system (Principal investigator)
- Extraction, identification and characterization of naturally occurring compounds and study of their biological activity on cell lines.
- Characterisation of extracellular vescicles
- Dynamical properties of proteins
Further collaborations:
- Antonella Bongiovanni (Cnr-Ibim)
- Antonio Cupane (Università degli studi di Palermo)
- Valeria Vetri (Università degli studi di Palermo)
- Judith Peters (ILL, Grenoble)
Financial support:
- FetOpen “Ves4Us - Extracellular vesicles from a natural source for tailormade Nanomaterials”
- Progetto FSE 2014/2020 “Percorsi formative nei settori Biotecnologia, Biomedicina e agroalimentare”
Key publications:
Costa MA, Mangione MR, Santonocito R, Passantino R, Giacomazza D, Librizzi F, Moran O, Carrotta R (2018) Biophysical characterization of asolectin-squalene liposomes. Colloids and Surface B: Biointerfaces 170:479-487
Nicastro MC, Spigolon D, Librizzi F, Moran O, Ortore MG, Bulone D, San Biagio PL, Carrotta R (2016) Amyloid beta-peptide insertion in liposomes containing GM1-cholesterol domains. BiophysChem 208:9–16. doi: 10.1016/j.bpc.2015.07.010
Librizzi F, Carrotta R, Spigolon D, Bulone D, San Biagio PL (2014) alfa-Casein Inhibits Insulin Amyloid Formation by Preventing the Onset of Secondary Nucleation Processes. J Phys Chem Lett 5:3043-3048. doi: 10.1021/jz501570m
Carrotta R, Vilasi S, Librizzi F, Martorana V, Bulone D, San Biagio PL (2012) alfa-Casein Inhibition Mechanism in Concanavalin A Aggregation Process. J Phys Chem B 116:14700-14707. doi: 10.1021/jp307417x
Carrotta R, Canale C, Diaspro A, Trapani A, San Biagio PL, Bulone D (2012) Inhibiting effect of alfas1-casein on Abeta1–40 fibrillogenesis. BBA 1820:124–132. doi: 10.1016/j.bbagen.2011.11.010
Publications (26):
Inhibition of AB1-42 Fibrillation by Chaperonins: Human Hsp60 Is a Stronger Inhibitor than Its Bacterial Homologue GroELVilasi S, Carrotta R, Ricci C, Rappa GC, Librizzi F, Martorana V, Ortore MG, Mangione MR
ACS chemical neuroscience, 2019
DOI: 10.1021/acschemneuro.9b00183
Amyloid beta-Peptide Interaction with Membranes: Can Chaperones Change the Fate?
Ricci C, Maccarini M, Falus P, Librizzi F, Mangione MR, Moran O, Ortore MG, Schweins R, Vilasi S, Carrotta R
The journal of physical chemistry. B, 2019
DOI: 10.1021/acs.jpcb.8b11719
Biophysical characterization of asolectin-squalene liposomes
Costa MA, Mangione MR, Santonocito R, Passantino R, Giacomazza D, Librizzi F, Moran O, Carrotta R
Colloids and surfaces. B, Biointerfaces (Print), 2018
DOI: 10.1016/j.colsurfb.2018.06.032
The effects of pressure on the energy landscape of proteins
Librizzi F, Carrotta R, Peters J, Cupane A
Scientific reports (Nature Publishing Group), 2018
DOI: 10.1038/s41598-018-20417-x
Investigation on different chemical stability of mitochondrial Hsp60 and its precursor
Ricci C, Carrotta R, Rappa GC, Mangione MR, Librizzi F, San Biagio PL, Amenitsch H, Ortore MG, Vilasi S
Biophysical chemistry (Print), 2017
DOI: 10.1016/j.bpc.2017.07.008
Amyloid beta-peptide insertion in liposomes containing GM1-cholesterol domains
Nicastro MC, Spigolon D, Librizzi F, Moran O, Ortore MG, Bulone D, Biagio PL, Carrotta R
Biophysical chemistry (Print), 2016
DOI: 10.1016/j.bpc.2015.07.010
Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins
Ricci, Caterina; Ortore, Maria Grazia; Vilasi, Silvia; Carrotta, Rita; Mangione, Maria Rosalia; Bulone, Donatella; Librizzi, Fabio; Spinozzi, Francesco; Burgio, Giosalba; Amenitsch, Heinz; San Biagio, Pier Luigi
Biophysical chemistry (Print), 2016
DOI: 10.1016/j.bpc.2015.07.006
Human Hsp60 with Its Mitochondrial Import Signal Occurs in Solution as Heptamers and Tetradecamers Remarkably Stable over a Wide Range of Concentrations
Vilasi, Silvia; Carrotta, Rita; Mangione, Maria Rosalia; Campanella, Claudia; Librizzi, Fabio; Randazzo, Loredana; Martorana, Vincenzo; Gammazza, Antonella Marino; Ortore, Maria Grazia; Vilasi, Annalisa; Pocsfalvi, Gabriella; Burgio, Giosalba; Corona, Davide; Piccionello, Antonio Palumbo; Zummo, Giovanni; Bulone, Donatella; de Macario, Everly Conway; Macario, Alberto J. L.; San Biagio, Pier Luigi; Cappello, Francesco
PloS one, 2014
DOI: 10.1371/journal.pone.0097657
alpha-Casein Inhibits Insulin Amyloid Formation by Preventing the Onset of Secondary Nucleation Processes
Librizzi, Fabio; Carrotta, Rita; Spigolon, Dario; Bulone, Donatella; San Biagio, Pier Luigi
The journal of physical chemistry letters, 2014
DOI: 10.1021/jz501570m
Different effects of Alzheimer's peptide A beta(1-40) oligomers and fibrils on supported lipid membranes
Canale, Claudio; Seghezza, Silvia; Vilasi, Silvia; Carrotta, Rita; Bulone, Donatella; Diaspro, Alberto; Biagio, Pier Luigi San; Dante, Silvia
Biophysical chemistry (Print), 2013
DOI: 10.1016/j.bpc.2013.07.010
Inhibiting effect of alpha(s1)-casein on A beta(1-40) fibrillogenesis
Carrotta R; Canale C; Diaspro A; Trapani A; San Biagio PL; Bulone D
Biochimica et biophysica acta. G, General subjects (Print), 2012
DOI: 10.1016/j.bbagen.2011.11.010
alpha-Casein Inhibition Mechanism in Concanavalin A Aggregation Process
Carrotta R; Vilasi S; Librizzi F; Martorana V; Bulone D; San Biagio PL
The journal of physical chemistry. B, 2012
DOI: 10.1021/jp307417x
Entrapment of A Beta 1-40 peptide in unstructured aggregates
Corsale C; Carrotta R; Mangione MR; Vilasi S; Provenzano A; Cavallaro G; Bulone D; San Biagio PL
Journal of physics. Condensed matter (Print), 2012
DOI: 10.1088/0953-8984/24/24/244103
Amyloid fibrils formation of concanavalin A at basic pH
Carrotta R (1); Vetri V(2); Librizzi F (2); Martorana V (1); Militello V(2); Leone M (2)
The journal of physical chemistry. B (1997 : Online), 2011
Insulin activated Akt rescues Ab oxidative stress induced cell death by orchestrating molecules trafficking
Picone P.; Giacomazza D.; Vetri V.; Carrotta R.; Militello V.; San Biagio P.L.; Di Carlo M.
Aging cell (Online), 2011
DOI: 10.1111/j.1474-9726.2011.00724.x
Concanavalin A aggregation and toxicity on cell cultures
Vetri V.; Carrotta R.; Picone P.; Di Carlo M.; Militello V.
Biochimica et biophysica acta. Proteins and proteomics, 2010
DOI: 10.1016/j.bbapap.2009.09.013
Concanavalin A aggregation and toxicity on cell cultures.
Vetri V; Carrotta R; Picone P; Di Carlo M; Militello V.
Biochimica et biophysica acta. Proteins and proteomics, 2010
DOI: 10.1016/j.bbapap.2009.09.013
Insulin promotes survival of amyloid-beta oligomers neuroblastoma damaged cells via caspase 9 inhibition and Hsp70 upregulation
Di Carlo, M., P. Picone, R. Carrotta, D. Giacomazza, P. L. San Biagio.
Journal of Biomedicine and Biotechnology (Online), 2010
DOI: 10.1155/2010/147835
Protein stability modulated by a conformational effector: effects of trifluoroethanol on bovine serum albumin.
Carrotta R; Manno M; Giordano FM; Longo A; Portale G; Martorana V; San Biagio PL.
PCCP. Physical chemistry chemical physics (Print), 2009
DOI: 10.1039/b818687a
The sea urchin embryo: A model to study Alzheimer's beta amyloid induced toxicity
M. Pellicanò; P. Picone; V. Cavalieri; R. Carrotta; G. Spinelli; M. Di Carlo
Archives of biochemistry and biophysics (Print), 2009
DOI: 10.1016/j.abb.2008.12.006
A beta oligomers and fibrillar aggregates induce different apoptotic pathways in LAN5 neuroblastoma cell cultures.
Picone P; Carrotta R; Montana G; Nobile MR; San Biagio PL; Di Carlo M.
Biophysical journal (Print), 2009
DOI: 10.1016/j.bpj.2008.11.056
Kinetics of different processes in human insulin amyloid formation.
Manno M.; Craparo E.F.; Podestà A.; Bulone D.; Carrotta R.; Martorana V.; Milani P.; Tiana G.; San Biagio P.L.
Journal of Molecular Biology, 2007
DOI: 10.1016/j.jmb.2006.11.008
Large size fibrillar bundles of the alzheimer amyloid beta-protein.
Carrotta R.; J. Barthès; A. Longo; V. Martorana; M. Manno; G. Portale; P. L. San Biagio.
European biophysics journal, 2007
DOI: 10.1007/s00249-007-0164-0
Employment of Cationic Solid-Lipid nanoparticles as RNA carriers
Montana G.; Bondì M.L.; Carrotta R.; Picone P.; Craparo E.F.; San Biagio P.L.; Giammona G.; Di Carlo M.
Bioconjugate chemistry, 2007
DOI: 10.1021/bc0601166
Toxicity of recombinant beta-amyloid prefibrillar oligomers on the morphogenesis of the sea urchin Paracentrotus lividus.
Carrotta R.; Di Carlo M.; Manno M.; Montana G.; Picone P.; Romancino D.; San Biagio P.L.
The FASEB journal, 2006
DOI: 10.1096/fj.06-5716fje
Protofibril formation of amyloid beta-protein at low pH via a non-cooperative elongation mechanism.
Carrotta R.; Manno M.; Bulone D.; Martorana V.; San Biagio P.L.;
The Journal of biological chemistry (Print), 2005
DOI: 10.1074/jbc.M500052200