Personal Card



Edi Gabellieri

Associate

1980 Degree in Biological Sciences – Pisa University
1981– 1990 Professional training fellowship (ex L. 285) at the Institute of Biophysics (PI)
From 1990 to now Researcher at the Institute of Biophysics – Section of Pisa

orcid://0000-0003-0750-4095,

Contact:

- Email edi.gabellieri@ibf.cnr.it
- Phone +39-050-3153048
- Location Pisa

Research interests:

- Interaction of proteins with nanoparticles (Principal investigator)

Key publications:

Elisabetta Morelli, Edi Gabellieri, Alessandra Bonomini, Danika Tognotti, Giacomo Grassi, Ilaria Corsi. 2018. TiO2 nanoparticles in seawater: aggregation and interactions with the green alga Dunaliella tertiolecta. Ecotoxicology and Environmental Safety 148 : 184-193.

V.V. Kulikova, E. A. Morozova, S. V. Revtovich, M. I. Kotlov, N. V. Anufrieva, N. P. Bazhulina, S. Raboni, S. Faggiano, E. Gabellieri, P. Cioni, Y. F. Belyi, A. Mozzarelli, T. V. Demidkina. 2017. Gene cloning, characterization and cytotoxic activity of methionine gamma-lyase from Clostridium novyi. IUBMB Life, 69:668-676.

E. Jacchetti, E. Gabellieri, P. Cioni, R. Bizzarri, R. Nifosì. 2016. Temperature and pressure effects on GFP mutants: explaining spectral changes by molecular dynamics simulation and TD-DFT calculations. Physical Chemistry Chemical Physics; 18: 12828-12838.

Patrizia Cioni, Edi Gabellieri, Stephane Marchal and Reinhard Lange. 2014. Temperature and pressure effects on C112S azurin: Volume, expansivity, and flexibility changes. Proteins 82:1787–1798.

E. Gabellieri, G.B. Strambini, D. Shcharbin, B. Klainert, M. Bryszewska 2006. Dendrimer-Protein Interactions Studied by Tryptophan Room Temperature Phosphorescence Biochim. Biophys. Acta 1764, 1750-1756.

Publications (45):

Exploiting gold nanoparticles for diagnosis and cancer treatments
D'Acunto Mario; Cioni Patrizia; Gabellieri Edi and Presciuttini Gianluca
Nanotechnology (Bristol. Print), 2021
DOI: 10.1088/1361-6528/abe1ed

Interplay between extracellular polymeric substances (EPS) from a marine diatom and model nanoplastic through eco-corona formation
Grassi Giacomo; Gabellieri Edi; Cioni Patrizia; Paccagnini Eugenio; Faleri Claudia; Lupetti Pietro; Corsi Ilaria and Morelli Elisabetta
Science of the total environment, 2020
DOI: 10.1016/j.scitotenv.2020.138457

TiO nanoparticles in seawater: Aggregation and interactions with the green alga Dunaliella tertiolecta
Morelli E, Gabellieri E, Bonomini A, Tognotti D, Grassi G, Corsi I
Ecotoxicology and environmental safety, 2018
DOI: 10.1016/j.ecoenv.2017.10.024

Engineering methionine ?-lyase from Citrobacter freundii for anticancer activity
Raboni S, Revtovich S, Demitri N, Giabbai B, Storici P, Cocconcelli C, Faggiano S, Rosini E, Pollegioni L, Galati S, Buschini A, Morozova E, Kulikova V, Nikulin A, Gabellieri E, Cioni P, Demidkina T, Mozzarelli A
Biochimica et biophysica acta. Proteins and proteomics, 2018
DOI: 10.1016/j.bbapap.2018.09.011

Soluble and Nanoporous Silica Gel-Entrapped C-freundii Methionine gamma-Lyase
Morozova EA, Kulikova VV, Faggiano S, Raboni S, Gabellieri E, Cioni P, Anufrieva NV, Revtovich SV, Demidkina T, Mozzarelli A
Journal of nanoscience and nanotechnology (Print), 2018
DOI: 10.1166/jnn.2018.14333

Gene cloning, characterization, and cytotoxic activity of methionine ?-lyase from Clostridium novyi
Kulikova, V.V. and Morozova, E.A. and Revtovich, S.V. and Kotlov, M.I. and Anufrieva, N.V. and Bazhulina, N.P. and Raboni, S. and Faggiano, S. and Gabellieri, E. and Cioni, P. and Belyi, Y.F. and Mozzarelli, A. and Demidkina, T.V.
IUBMB life (Print), 2017
DOI: 10.1002/iub.1649

Temperature and pressure effects on GFP mutants: Explaining spectral changes by molecular dynamics simulations and TD-DFT calculations
Jacchetti E.; Gabellieri E.; Cioni P.; Bizzarri R.; Nifosi R.
PCCP. Physical chemistry chemical physics (Print), 2016
DOI: 10.1039/c6cp01274d

The response of Phaeodactylum tricornutum to quantum dot exposure: Acclimation and changes in protein expression.
Morelli E., Salvadori E., Basso B., Tognotti D., Cioni P., Gabellieri E.
Marine environmental research, 2015
DOI: 10.1016/j.marenvres.2015.06.018

A SELDI-TOF approach to ecotoxicology: Comparative profiling of low molecular weight proteins from a marine diatom exposed to CdSe/ZnS quantum dots
Scebba F.; Tognotti D.; Presciuttini G.; Gabellieri E.; Cioni P.; Angeloni D.; Basso B.; Morelli E.
Ecotoxicology and environmental safety (Online), 2015
DOI: 10.1016/j.ecoenv.2015.08.024

Temperature and pressure effects on C112S azurin: Volume, expansivity, and flexibility changes
Cioni P.; Gabellieri E.; Marchal S.; Lange R.
Proteins (Online), 2014
DOI: 10.1002/prot.24532

Interaction of CdSe/ZnS quantum dots with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta: A biophysical approach
Morelli E, Salvadori E, Bizzarri R, Cioni P, Gabellieri E
Biophysical chemistry (Print), 2013
DOI: 10.1016/j.bpc.2013.06.007

Temperature and pressure dependence of azurin stability as monitored by tryptophan fluorescence and phosphorescence. The case of F29A mutant
Tognotti D, Gabellieri E, Morelli E, Cioni P
Biophysical chemistry (Print), 2013
DOI: 10.1016/j.bpc.2013.06.005

Nitrogen fertilizer improves boron phytoextraction by Brassica juncea grown in contaminated sediments and alleviates plant stress
Giansoldati V; Tassi E; Morelli E; Gabellieri E; Pedron F; Barbafieri M
Chemosphere, 2012
DOI: 10.1016/j.chemosphere.2012.02.005

Chemical stability of CdSe quantum dots in seawater and their effects on a marine microalga
Morelli E; Cioni P; Posarelli M; Gabellieri E
Aquatic toxicology, 2012
DOI: 10.1016/j.aquatox.2012.06.012

Protein dynamics and pressure: what can high pressure tell us about protein structural flexibility?
Cioni P, Gabellieri E
Biochimica et biophysica acta. Proteins and proteomics, 2011
DOI: 10.1016/j.bbapap.2010.09.017

Does azurin bind to the transactivation domain of p53? A Trp phosphorescence study
Gabellieri E, Bucciantini M, Stefani M, Cioni P
Biophysical chemistry (Print), 2011

Protein structural changes induced by glutathione-coated CdS quantum dots as revealed by Trp phosphorescence
Gabellieri E, Cioni P, Balestreri E, Morelli E
European biophysics journal, 2011
DOI: 10.1007/s00249-011-0736-x

Cavity-Creating Mutations in Pseudomonas aeruginosa Azurin: Effects on Protein Dynamics and Stability.
Gabellieri E, Balestreri E, Galli A., and P Cioni.
Biophysical journal (Print), 2008

ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in Ice
Gabellieri E., Strambini G.
Biophysical journal (Print), 2006
DOI: 10.1529/biophysj.105.074948

Dendrimer-Protein Interactions Studied by Tryptophan Room Temperature Phosphorescence
Gabellieri E., G.B. Strambini, D. Shcharbin, B. Klainert, M. Bryszewska
Biochimica et biophysica acta. Proteins and proteomics, 2006
DOI: 10.1016/j.bbapap.2006.09.008

Substrate-induced Conformational Changes in the Membrane-embedded IICmtl-domain of the Mannitol Permease from Escherichia coli, EnzymeIImtl, Probed by Tryptophan Phosphorescence Spectroscopy.
Veldhuis G.; Gabellieri E.; Vos Erwin P.P.; Poolman B.; Strambini G.B; Broos J.
The Journal of biological chemistry (Print), 2005

Efficient biosynthetic incorporation of tryptophan and indole analogs in an integral membrane protein
Broos J., Gabellieri E., Biemans-Oldehinkel E., Strambini G.B.
Protein science (Print), 2003

Perturbation of protein tertiary structure in frozen solutions revealed by 1-Anilino-8-Naphthalene Sulfonate fluorescence
Gabellieri E. 1, Strambini G.B. 1
Biophysical journal (Print), 2003

Tryptophan phosphorescence spectroscopy reveals that a domain in the NAD(H)-binding component (dI) of transhydrogenase from Rhodospirillum rubrum, has an extremely rigid and conformationally homogeneous protein core
Broos J., Gabellieri E., Van Boxel G.I., Jackson J.B., Strambini G.B.

Structural Perturbations of Azurin Deposited on Solid Matrices as Revealed by Trp Phosphorescence
Gabellieri E. 1, Strambini G.B. 1
Biophysical journal (Print), 2001

Alteration of the intramolecular dynamics of glycogen phosphorylase b by allosteric ligands
Szarka, Á.b and Gabellieri, E.c and Gonnelli, M.c and Cioni, P.c and Lakos, Z.a and Somogyi, B.a
Journal of photochemistry and photobiology. B, Biology (Print), 1998
DOI: 10.1016/S1011-1344(97)00119-X

Modification of the mitochondrial F1-ATPase epsilon subunit, enhancement of the ATPase activity of the IF1-F1 complex and IF1-binding dependence of the conformation of the epsilon subunit.
Solaini, G. and Baracca, A. and Gabellieri, E. and Lenaz, G.
Biochemical journal (Lond., 1984), 1997

Structural mapping of the gamma-subunit of mitochondrial H+-ATPase complex (F1)
Gabellieri, E.a and Strambini, G.B.a and Baracca, A.b and Solaini, G.c d
Biophysical journal (Print), 1997

Effects of NAD+ binding on the luminescence of tryptophans 84 and 310 of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus
Gabellieri, E.a and Rahuel-Clermont, S.b and Branlant, G.b and Strambini, G.B.a
Biochemistry (Easton), 1996
DOI: 10.1021/bi960231b

Proteins in frozen solutions: Evidence of ice-induced partial unfolding
Strambini, G.B. and Gabellieri, E.
Biophysical journal (Print), 1996

Conformational changes of the mitochondrial F1-ATPase ?-subunit induced by nucleotide binding as observed by phosphorescence spectroscopy
Baracca, A.a and Gabellieri, E.b and Barogi, S.a and Solaini, G.c
The Journal of biological chemistry (Print), 1995

A study of the mitochondrial F1-ATPase tryptophan phosphorescence at 273 K
Baracca, A.a and Barogi, S.a and Gabellieri, E.b and Lenaz, G.a and Solaini, G.c d
Biochemical and biophysical research communications (Print), 1995
DOI: 10.1006/bbrc.1995.1197

Conformational changes in proteins induced by dynamic associations. A tryptophan phosphorescence study
Gabellieri, E. and Strambini, G.B.
European journal of biochemistry (Print), 1994

Heterogeneity of protein conformation in solution from the lifetime of tryptophan phosphorescence
Cioni, P. and Gabellieri, E. and Gonnelli, M. and Strambini, G.B.
Biophysical chemistry (Print), 1994

Quenching of indole luminescence by copper ions: A distance dependence study
Strambini, G.B. and Gabellieri, E.
Journal of physical chemistry (1952), 1991

Phosphorescence from Trp-48 in azurin: Influence of Cu(II), Cu(I), Ag(I), and Cd(II) at the coordination site
Strambini, G.B. and Gabellieri, E.
Journal of physical chemistry (1952), 1991

Temperature dependence of tryptophan phosphorescence in proteins.
Strambini, G.B. and Gabellieri, E.
Photochemistry and photobiology, 1990

Phosphorescence properties of Trp-84 and Trp-310 in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus
Gabellieri, E. and Strambini, G.B.
Biophysical chemistry (Print), 1989
DOI: 10.1016/0301-4622(89)80027-4

Phosphorescence properties and protein structure surrounding tryptophan residues in yeast, pig, and rabbit glyceraldehyde-3-phosphate dehydrogenase
Strambini, G.B. and Gabellieri, E.
Biochemistry (Easton), 1989

Tryptophan phosphorescence and the conformation of liver alcohol dehydrogenase in solution and in the crystalline state
Gabellieri, E. and Strambini, G.B. and Gualtieri, P.
Biophysical chemistry (Print), 1988

Phosphorescence anisotropy of liver alcohol dehydrogenase in the crystalline state. Apparent glasslike rigidity of the coenzyme-binding domain
Strambini, G.B. and Gabellieri, E.
Biochemistry (Easton), 1987

Relationship of disulfide bonds to the maintenance of the active secondary structure of alfalfa (Medicago sativa ) leaves protease inhibitor
Gonnelli, M. and Balestreri, E. and Romagnoli, A. and Fissi, A. and Cioni, P. and Gabellieri, E. and Felicioli, R.
Journal of agricultural and food chemistry, 1986

Purification and characterization of two leaf polypeptide inhibitors of leaf protease from alfalfa (Medicago sativa)
Gonnelli, M.a and Cioni, P.a and Romagnoli, A.a and Gabellieri, E.a and Balestreri, E.a and Felicioli, R.b
Archives of biochemistry and biophysics (Print), 1985

Intrinsic phosphorescence from proteins in the solid state.
Strambini, G.B. and Gabellieri, E.
Photochemistry and photobiology, 1984

Complementing S-peptide as modulator in enzyme immunoassay
Margherita Gonnelli, Edi Gabellieri, Giorgio Montagnoli, Romano Felicioli
Biochemical and biophysical research communications (Print), 1981