Anno2015
AutoriPodobnik, Marjetka; Marchioretto, Marta; Zanetti, Manuela; Bavdek, Andrej; Kisovec, Matic; Cajnko, Misa Mojca; Lunelli, Lorenzo; Dalla Serra, Mauro; Anderluh, Gregor
AbstractPore formation of cellular membranes is an ancient mechanism of bacterial pathogenesis that allows efficient damaging of target cells. Several mechanisms have been described, however, relatively little is known about the assembly and properties of pores. Listeriolysin O (LLO) is a pH-regulated cholesterol-dependent cytolysin from the intracellular pathogen Listeria monocytogenes, which forms transmembrane beta-barrel pores. Here we report that the assembly of LLO pores is rapid and efficient irrespective of pH. While pore diameters at the membrane surface are comparable at either pH 5.5 or 7.4, the distribution of pore conductances is significantly pH-dependent. This is directed by the unique residue H311, which is also important for the conformational stability of the LLO monomer and the rate of pore formation. The functional pores exhibit variations in height profiles and can reconfigure significantly by merging to other full pores or arcs. Our results indicate significant plasticity of large beta-barrel pores, controlled by environmental cues like pH.
RivistaScientific Reports (nature Publishing Group)
ISSN2045-2322
Impact factor0
Volume5
Pagina inizio1
Pagina fine10
Autori IBFMauro DALLA SERRA, Manuela ZANETTI, Lorenzo LUNELLI, Marta MARCHIORETTO
Linee di Ricerca IBFMD.P01.028.001
Sedi IBFIBF.TN