Anno2015
AutoriHorrocks M.H.; Tosatto L.; Dear A.J.; Garcia G.A.; Iljina M.; Cremades N.; Dalla Serra M.; Knowles T.P.J.; Dobson C.M.; Klenerman D.
Abstract?-Synuclein oligomers can be toxic to cells and may be responsible for cell death in Parkinson's disease. Their typically low abundance and highly heterogeneous nature, however, make such species challenging to study using traditional biochemical techniques. By combining fast-flow microfluidics with single-molecule fluorescence, we are able to rapidly follow the process by which oligomers of ?S are formed and to characterize the species themselves. We have used the technique to show that populations of oligomers with different FRET efficiencies have varying stabilities when diluted into low ionic strength solutions. Interestingly, we have found that oligomers formed early in the aggregation pathway have electrostatic repulsions that are shielded in the high ionic strength buffer and therefore dissociate when diluted into lower ionic strength solutions. This property can be used to isolate different structural groups of ?S oligomers and can help to rationalize some aspects of ?S amyloid fibril formation. (Figure Presented).
RivistaAnalytical Chemistry (online)
ISSN1520-6882
Impact factor0
Volume87
Pagina inizio8818
Pagina fine8826
Autori IBFMauro DALLA SERRA, Laura TOSATTO
Linee di Ricerca IBFMD.P01.028.001
Sedi IBFIBF.TN