Anno2015
AutoriDellafiora L.; Marchetti M.; Spyrakis F.; Orlandi V.; Campanini B.; Cruciani G.; Cozzini P.; Mozzarelli A.
AbstractSerine racemase, the enzyme responsible for D-serine synthesis in the central nervous system, has been identified as a potential therapeutic target to treat N-methyl-D-aspartate receptors-related pathologies. The search for specific inhibitors of the enzyme has revealed that serine racemase is a difficult target, with the best inhibitor currently identified, 2,2-dichloromalonate, showing a K-i of 19 mu M. In order to expand the chemical space of hit compounds, we have performed an in silico structure-based screening campaign on a filtered ZINC library applying the FLAP software. The identified hits were docked with GOLD and re-scored with HINT, and the most promising molecules experimentally evaluated on recombinant human serine racemase. Two inhibitors, with chemical structures totally unrelated to inhibitors described so far showed K-i values of about 1.5 mM.
RivistaBioorganic & Medicinal Chemistry Letters (print)
ISSN0960-894X
Impact factor0
Volume25
Pagina inizio4297
Pagina fine4303
Autori IBFAndrea MOZZARELLI
Linee di Ricerca IBFMD.P01.008.001
Sedi IBFIBF.PI