AutoriBoccaccio Anna;Sagheddu C;Menini Anna
AbstractPhotolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active
compounds1. Caged compounds are molecules made physiologically inactive by a chemical cage that can be broken by a flash of ultraviolet light.
Here, we show how to obtain patch-clamp recordings combined with photolysis of caged compounds for the study of olfactory transduction in
dissociated mouse olfactory sensory neurons. The process of olfactory transduction (Figure 1) takes place in the cilia of olfactory sensory
neurons, where odorant binding to receptors leads to the increase of cAMP that opens cyclic nucleotide-gated (CNG) channels2. Ca entry through
CNG channels activates Ca-activated Cl channels. We show how to dissociate neurons from the mouse olfactory epithelium3 and how to activate
CNG channels or Ca-activated Cl channels by photolysis of caged cAMP4 or caged Ca5. We use a flash lamp6,7 to apply ultraviolet flashes to the
ciliary region to uncage cAMP or Ca while patch-clamp recordings are taken to measure the current in the whole-cell voltage-clamp configuration
RivistaJournal Of Visualized Experiments
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Pagina inizioe3195
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Autori IBFAnna Elisabetta BOCCACCIO
Linee di Ricerca IBFMD.P01.001.001