Anno2014
AutoriClamer M.; Hofler L.; Mikhailova E.; Viero G.; Bayley H.
AbstractPost-transcriptional modifications of the 3?-ends of RNA molecules have a profound impact on their stability and processing in the cell. Uridylation, the addition of uridines to 3?-ends, has recently been found to be an important regulatory signal to stabilize the tagged molecules or to direct them toward degradation. Simple and cost-effective methods for the detection of this post-transcriptional modification are not yet available. Here, we demonstrate the selective and transient binding of 3?-uridylated ssRNAs inside the ? barrel of the staphylococcal ?-hemolysin (?HL) nanopore and investigate the molecular basis of uridine recognition by the pore. We show the discrimination of 3?-oligouridine tails on the basis of their lengths and propose the ?HL nanopore as a useful sensor for this biologically relevant RNA modification. © 2013 American Chemical Society.
RivistaAcs Nano (online)
ISSN1936-086X
Impact factor0
Volume8
Pagina inizio1364
Pagina fine1374
Autori IBFGabriella VIERO
Linee di Ricerca IBFMD.P01.028.001
Sedi IBFIBF.TN