AutoriC. Dispenza, S. Rigogliuso, N. Grimaldi, M.A. Sabatino,D. Bulone, M.L. Bondì, G. Ghersi
AbstractAminopropyl methacrylamide chloride-graft-poly(N-vinyl pyrrolidone) nanogels (NGs) weredesigned to exploit the favorable properties of poly(N-vinyl pyrrolidone) (PVP), such as its highaffinity to water and complexation ability of ions, molecules and macromolecules, with theavailability of primary amino groups for bioconjugation reactions. A thorough structuralcharacterization of the nanoscalar networks was performed via 1H-NMR and solid state 13C-NMRspectroscopies, while solid state NMR relaxation time measurements completed the NGsdescription in terms of polymer network density. Information on the hydrodynamic size and surfacecharge densities were sought via dynamic light scattering (DLS) and ?-potential measurements.These measurements, carried out both in water and in buffer and at the variance of pH, temperatureand time, support the excellent colloidal stability of the amino-graft PVP nanogels and theirredispersability from the dry physical form. The total absence of in-vitro toxicity andimmunogenicity was ascertained first, and then cell localization studies in cell cultures offluorescent variants were performed. Furthermore, the possibility of decorating the nanogels withbovine serum albumin (BSA) to obtain a stable bio-hybrid colloidal nanoconstruct was established.All the gathered evidences qualify these materials as potential candidates to be used as nanocarriersfor intra-cellular delivery of protein and other biomolecular drugs
RivistaReactive & Functional Polymers
Impact factor
Pagina inizio1103
Pagina fine1113
Autori IBFDonatella BULONE, Clelia DISPENZA
Linee di Ricerca IBFMD.P01.002.001