Anno2013
AutoriRomani G, Piotrowski A, Hillmer S, Gurnon J, Van Etten JL, Moroni A, Thiel G, Hertel B
AbstractMost chloroviruses encode small K+ channels, which are functional in electrophysiological assays. The experimental finding that initial steps in viral infection exhibit the same sensitivity to channel inhibitors as the viral K+ channels has led to the hypothesis that the channels are structural proteins located in the internal membrane of the virus particles. This hypothesis was questioned recently because proteomic studies failed to detect the channel protein in virions of the prototype chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1). Here, we used a mAb raised against the functional K+ channel from chlorovirus MA-1D to search for the viral K+ channel in the virus particle. The results showed that the antibody was specific and bound to the tetrameric channel on the extracellular side. The antibody reacted in a virus-specific manner with protein extracts from chloroviruses that encoded channels similar to that from MA-1D. There was no cross-reactivity with chloroviruses that encoded more diverse channels or with a chlorovirus that lacked a K+ channel gene. Together with electron microscopic imaging, which revealed labelling of individual virus particles with the channel antibody, these results establish that the viral particles contain an active K+ channel, presumably located in the lipid membrane that surrounds the DNA in the mature virions.
RivistaJournal Of General Virology
ISSN0022-1317
Impact factor
Volume94
Pagina inizio2549
Pagina fine2556
Autori IBFGiulia Angelica Maria ROMANI, Anna MORONI
Linee di Ricerca IBFMD.P01.005.001
Sedi IBFIBF.MI