Anno2013
AutoriFrassanito AM, Barsanti L, Passarelli V, Evangelista V, Gualtieri P
AbstractHere we report the identification and expression of a second rhodopsin-like protein in the alga Cyanophora paradoxa (Glaucophyta), named Cyanophopsin_2. This new protein was identified due to a serendipity event, since the RACE reaction performed to complete the sequence of Cyanophopsin_1, (the first rhodopsin-like protein of C. paradoxa identified in 2009 by our group), amplified a 619 bp sequence corresponding to a portion of a new gene of the same protein family. The full sequence consists of 1175 bp consisting of 849 bp coding DNA sequence and 4 introns of 326 bp. The protein is characterized by an N-terminal region of 47 amino acids, followed by a region with 7 ?-helices of 213 amino acids and a C-terminal region of 22 amino acids. This protein showed high identity with Cyanophopsin_1 and other rhodopsin-like proteins of Archea, Bacteria, Fungi and Algae. Cyanophosin_2 (CpR2) was expressed in a cell-free expression system, and characterized by means of absorption spectroscopy.
RivistaJournal Of Photochemistry And Photobiology. B - Biology
ISSN
Impact factor
Volume125
Pagina inizio188
Pagina fine193
Autori IBFValtere EVANGELISTA, Vincenzo PASSARELLI, Laura BARSANTI, Paolo GUALTIERI, Anna Maria FRASSANITO
Linee di Ricerca IBFMD.P01.020.001
Sedi IBFIBF.PI