Anno2013
AutoriZanardi I, Zifarelli G, Pusch M
AbstractOsteoporosis, characterized by excessive osteoclast mediated bone resorption, affects millions of people worldwide representing a major public health problem. ClC-7 is a chloride-proton exchanger localized in lysosomes and in the resorption lacuna in osteoclasts where it is essential for bone resorption. Thus, drugs targeted at ClC-7 have been proposed for ameliorating osteoporosis. However, functional assays suited for high throughput screening (HTS) of ClC-7 function are lacking. Here we describe two complementary variants of purely optical assays of the transport activity of ClC-7, redirected to the plasma membrane employing a genetically encoded fluorescent Cl-/pH indicator fused to the ClC-7 protein. These simple and robust functional assays of ClC-7 transport are well-suited to be applied in HTS of small-molecule inhibitors and may help to develop drugs suited for the treatment of osteoporosis.
RivistaScientific Reports
ISSN
Impact factor
Volume3
Pagina inizio
Pagina fine
Autori IBFMichael PUSCH, Giovanni ZIFARELLI, Ilaria ZANARDI
Linee di Ricerca IBFMD.P01.009.001
Sedi IBFIBF.GE