Anno2013
AutoriPasquardini L; Berneschi S; Barucci A; Cosi F; Dallapiccola R; Insinna M; Lunelli L; Conti GN; Pederzolli C; Salvadori S; Soria S
AbstractWhispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
RivistaJournal Of Biophotonics
ISSN
Impact factor
Volume6
Pagina inizio178
Pagina fine187
Autori IBFLorenzo LUNELLI
Linee di Ricerca IBFMD.P01.028.001
Sedi IBFIBF.TN