Anno2010
AutoriApolloni S, Finocchi P, D’Agnano I, Alloisio S, Nobile M, D’Ambrosi N, Volonté C
AbstractThe role of P2 receptors for purines/pyrimidines is not well characterized in neuroblastoma, although a variety of purinergic mRNAs/proteins are expressed in these cells. Among these, the P2Y(6) receptor is the only subtype distinguished by UDP-specific activation. In this work, after over-expressing the P2Y(6) protein in human neuroblastoma SH-SY5Y cells, we find that UDP arrests cell cycle and induces apoptosis, by counteracting the pathological functioning of neuroblastoma in vitro. UDP also causes mitochondrial damage through diffusion of cytochrome c in the cytoplasm, and stimulates caspase-3,7,8 activities, with extensive over-expression of manganese superoxide dismutase. Our data establish the direct toxic role and anti-cancer activity of UDP in a neuroblastoma cell line, and identify the P2Y(6) receptor as a novel potential target in anti-tumoural therapies. This constitutes an advancement not only in the knowledge of purinergic signalling, but also in the biological and pathological aspects of neuroblastoma in vitro.
RivistaNeurochemistry International
ISSN
Impact factor
Volume56
Pagina inizio670
Pagina fine678
Autori IBFMario NOBILE, Susanna ALLOISIO
Linee di Ricerca IBFMD.P01.001.001
Sedi IBFIBF.GE