Anno2010
AutoriArosio D, Ricci F, Marchetti L, Gualdani R, Albertazzi L, Beltram F
AbstractChloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.
RivistaNature Methods
ISSN
Impact factor
Volume7
Pagina inizio516
Pagina fine518
Autori IBFDaniele AROSIO
Linee di Ricerca IBFMD.P01.001.001
Sedi IBFIBF.TN