AutoriSusanna Alloisio(a); Chiara Cervetto(b); Mario Passalacqua(c); Raffaella Barbieri(a); Guido Maura(b,d,e); Mario Nobile(a); Manuela Marcoli(b)
AbstractThe presynaptic P2X7 receptor (P2X7R) plays an important role in the modulation of transmitter release. We recently demonstrated that, in nerve terminals of the adult rat cerebral cortex, P2X7R activation induced Ca2+-dependent vesicular glutamate release and significant Ca2+-independent glutamate efflux through the P2X7R itself. In the present study, we investigated the effect of the new selective P2X(7)R competitive antagonist 3-(5-(2,3-dichlorophenyl)-1H-tetrazol-1-yl)methyl pyridine (A-438079) on cerebrocortical terminal intracellular calcium (intrasynaptosomal calcium concentration;[Ca2+](i) signals and glutamate release, and evaluated whether P2X7R immunoreactivity was consistent with these functional tests. A-438079 inhibited functional responses. P2X7R immunoreactivity was found in about 45% of cerebrocortical terminals, including glutamatergic and non-glutamatergic terminals. This percentage was similar to that of synaptosomes showing P2X7R-mediated [Ca2+]i signals. These findings provide compelling evidence of functional presynaptic P2X7R in cortical nerve terminals.
RivistaFebs Letters (print)
Impact factor
Pagina inizio3948
Pagina fine3953
Autori IBFMario NOBILE, Susanna ALLOISIO, Raffaella BARBIERI
Linee di Ricerca IBFMD.P01.001.001