|Autori||Coluccino L, Stagnaro P, Vassalli M, Scaglione S|
|Abstract||Background: The design of an appropriate microenvironment for stem cell differentiation constitutes a multitask |
mission and a critical step toward the clinical application of tissue substitutes. With the aim of producing a bioactive
material for orthopedic applications, a transforming growth factor-? (TGF- ?1)/hydroxyapatite (HA) association
within an alginate-based scaffold was investigated. The bioactive scaffold was carefully designed to offer
specific biochemical cues for an efficient and selective cell differentiation toward the bony and chondral lineages.
Methods: Highly porous alginate scaffolds were fabricated from a mixture of calcium cross-linked alginates by
means of a freeze-drying technique. In the chondral layer, the TGF in citric acid was mixed with an alginate/
alginate-sulfate solution. In the bony layer, HA granules were added as bioactive signal, to offer an osteoinductive
surface to the cells. Optical and scanning electron microscopy analyses were performed to assess the macromicro
architecture of the biphasic scaffold. Different mechanical tests were conducted to evaluate the elastic
modulus of the grafts. For the biological validation of the developed prototype, mesenchymal stem cells were
loaded onto the samples; cellular adhesion, proliferation and in vivo biocompatibility were evaluated.
Results and conclusions: The results successfully demonstrated the efficacy of the designed osteochondral graft,
which combined interesting functional properties and biomechanical performances, thus becoming a promising
candidate for osteochondral tissue-engineering applications.
|Rivista||J Appl Biomater Function Mater|
|Autori IBF||Massimo VASSALLI|
|Linee di Ricerca IBF||MD.P01.001.001|