| Sigla | MD.P01.009.001 |
| Inizio | 2009 |
| Termine | 2014 |
| Commessa | MD.P01.009 |
| Responsabile | Michael Pusch |
| Tematiche di ricerca | Le tematiche della commessa comprendono l'analisi della relazione tra struttura e funzione di canali/recettori postsinaptici glutamatergici e di canali e trasportatori del tipo CFTR e CLC. Per i canali/recettori glutamatergici viene inoltre studiata l'interazione con metalli pesanti la cui tossicita’ si esplica mediante un’interazione diretta del metallo con la proteina canale con conseguente alterazione delle caratteristiche funzionali. Per quanto riguarda i canali CLC ci si propone di approfondire i meccanismi molecolari del funzionamento, in considerazione soprattutto delle importanti funzioni fisiologiche svolte e del loro coinvolgimento in almeno cinque patologie genetiche diverse. Per le proteine CFTR e CLC vengono sviluppati e studiati nuovi strumenti farmacologici di interesse per il trattamento delle malattie genetiche, in particolare per la fibrosi cistica, che e’ la malattia genetica letale piu’ frequente. Sono sempre in sviluppo nuove tecnologie e metodi. |
| Personale di Ricerca (14) | ||
|---|---|---|
| Nome COGNOME | Struttura | Ruolo |
| Debora Baroni | IBF-GE | Personale collaboratore |
| Gian Piero Borello | IBF-GE | Personale di ricerca |
| Franco Conti | IBF-GE | Personale associato |
| Silvia De Stefano | IBF-GE | Personale collaboratore |
| Michele Fiore | IBF-GE | Personale collaboratore |
| Paola Gavazzo | IBF-GE | Personale di ricerca |
| Antonella Gradogna | IBF-GE | Personale collaboratore |
| Elena Jeworutzki | IBF-GE | Personale collaboratore |
| Carla Marchetti | IBF-GE | Personale di ricerca |
| Oscar Moran | IBF-GE | Personale di ricerca |
| Michael Pusch | IBF-GE | Personale di ricerca |
| Francesca Spano' | IBF-GE | Personale di ricerca |
| Ilaria Zanardi | IBF-GE | Personale collaboratore |
| Giovanni Zifarelli | IBF-GE | Personale di ricerca |
| Pubblicazioni (33) | |
|---|---|
| Anno | Autori/Titolo/Rivista |
| 2012 | Ruivo R, Bellenchi GC, Chen X, Zifarelli G, Sagne' C, Debacker C, Pusch M, Supplisson S, Gasnier B. Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin Proceedings Of The National Academy Of Sciences Of The United States Of America |
| 2012 | Costa A; Gutla PVK; Boccaccio A; Scholz-Starke J; Festa M; Basso B; Zanardi I; Pusch M; Lo Schiavo F; Gambale F; Carpaneto A The Arabidopsis central vacuole as an expression system for intracellular transporters: functional characterization of the Cl-/H+ exchanger CLC-7 Journal Of Physiology |
| 2012 | Jeworutzki E; Lopez-Hernandez T; Capdevila-Nortes X; Bengtsson L; Sirisi S; Montolio M; Zifarelli G; Arnedo T; Muller CS; Schulte U; Nunes V; Martinez A; Jentsch TJ; Gasull X; Pusch M; Estevez R GlialCAM, a Protein Defective in a Leukodystrophy, Serves as a CIC-2 Cl- Channel Auxiliary Subunit Neuron |
| 2012 | Mescola A; Vella S; Scotto M; Gavazzo P; Canale C; Diaspro A; Pagano A; Vassalli M Probing cytoskeleton organisation of neuroblastoma cells with single-cell force spectroscopy Jmr. Journal Of Molecular Recognition |
| 2012 | Baranowska-Bosiacka, Irena; Gutowska, Izabela; Marchlewicz, Mariola; Marchetti, Carla; Kurzawski, Mateusz; Dziedziejko, Viotetta; Kolasa, Agnieszka; Olszewska, Maria; Rybicka, Marta; Safranow, Krzysztof; Nowacki, Przemyslaw; (Wiszniewska, Barbara; Chlubek Disrupted pro- and antioxidative balance as a mechanism of neurotoxicity induced by perinatal exposure to lead Brain Research |
| 2012 | Zifarelli G., De Stefano S., Zanardi I., Pusch M. On the mechanism of gating charge movement of ClC-5, a human Cl(-)/H(+) antiporter Biophysical Journal |
| 2012 | Galfre' E; Galeno L.; Moran O. A potentiator induces conformational changes on the recombinant CFTR nucleotide binding domains in solution. Cellular And Molecular Life Sciences |
| 2012 | Marasini, C.; Galeno, L.; Moran, O. Thermodynamic study of the native and phosphorylated regulatory domain of the CFTR Biochemical And Biophysical Research Communications |
| 2012 | Barbieri R; Baroni D; Moran O Identification of an intra-molecular disulfide bond in the sodium channel beta 1-subunit Biochemical And Biophysical Research Communications |
| 2012 | Baroni D; Moran O Molecular differential expression of voltage-gated sodium channel alpha and beta subunit mRNAs in five different mammalian cell lines Journal Of Bioenergetics And Biomembranes |
| 2012 | Bruzzone MJ; Gavazzo P; Massone S; Balbi C; Villa F; Conti A; Dieci G; Cancedda R; Pagano A The Murine PSE/TATA-Dependent Transcriptome: Evidence of Functional Homologies with Its Human Counterpart International Journal Of Molecular Sciences |
| 2011 | Strutz-Seebohm N, Pusch M, Wolf S, Stoll R, Tapken D, Gerwert K, Attali B, Seebohm G Structural basis of slow activation gating in the cardiac I Ks channel complex Cellular Physiology And Biochemistry (10099j0) |
| 2011 | Gavazzo P, Guida P, Marchetti C The influence of Calcium ions on Nickel modulation of NMDA receptor currents Metallomics (00599nn) |
| 2011 | Baranowska-Bosiacka I, Gutowska I, Marchetti C, Rutkowska M, Marchlewicz M, Kolasa A, Prokopowicz A, Wiernicki I, Piotrowska K, Bakiewicz M, Safranow K, Wiszniewska B, Chlubek D Altered energy status of primary cerebellar granule neuronal cultures from rats exposed to lead in the pre- and neonatal period Toxicology (92455j0) |
| 2011 | Gavazzo P, Tedesco M, Chiappalone M, Zanardi I, Marchetti C Nickel modulates the electrical activity of cultured cortical neurons through a specific effect on N-methyl-d-aspartate receptor channels Neuroscience (71612j0) |
| 2011 | Gavazzo P, Vella S, Marchetti C, Nizzari M, Cancedda R, Pagano A Acquisition of neuron-like electrophysiological properties in neuroblastoma cells by controlled expression of NDM29 ncRNA Journal Of Neurochemistry (58124j0) |
| 2011 | De Stefano S, Pusch M, Zifarelli G Extracellular Determinants of Anion Discrimination of the Cl-/H+ Antiporter Protein CLC-5 Journal Of Biological Chemistry (51172j0) |
| 2011 | Baroni D, Moran O Molecular differential expression of voltage-gated sodium channel alpha and beta subunit mRNAs in five different mammalian cell lines Journal Of Bioenergetics And Biomembranes (51158j0) |
| 2011 | Galeno L, Galfr? E, Moran O Small-angle X-ray scattering study of the ATP modulation of the structural features of the nucleotide binding domains of the CFTR in solution European Biophysics Journal With Biophysics Letters (05881j0) |
| 2011 | Marchetti C, Gavazzo P, Stafford GI, Van Staden J South African plants used in traditional medicine to treat epilepsy have an antagonistic effect on NMDA receptor currents Journal Of Ethnopharmacology (01583j0) |
| 2010 | Gradogna A, Babini E, Picollo A, Pusch M A regulatory calcium-binding site at the subunit interface of CLC-K kidney chloride channels Journal Of General Physiology (54824j0) |
| 2010 | Zifarelli G, Pusch M CLC transport proteins in plants Febs Letters (37070j0) |
| 2010 | Zifarelli G, Liantonio A, Gradogna A, Picollo A, Gramegna G, De Bellis M, Murgia AR, Babini E, Camerino DC, Pusch M Identification of sites responsible for the potentiating effect of niflumic acid on ClC-Ka kidney chloride channels. British Journal Of Pharmacology (22022j0) |
| 2010 | Moran O Model of the cAMP activation of chloride transport by CFTR channel and the mechanism of potentiators. Journal Of Theoretical Biology (62392j0) |
| 2010 | Pangrazio A, Pusch M, Caldana E, Frattini A, Lanino E, Tamhankar PM, Phadke S, Lopez AG, Orchard P, Mihci E, Abinun M, Wright M, Vettenranta K, Bariae I, Melis D, Tezcan I, Baumann C, Locatelli F, Zecca M, Horwitz E, Mansour LS, Van Roij M, Vezzoni P Molecular and clinical heterogeneity in CLCN7-dependent osteopetrosis: report of 20 novel mutations. Human Mutation (10012j0) |
| 2010 | Bisignano P, Moran O Molecular dynamics analysis of the wild type and dF508 mutant structures of the human CFTR-nucleotide binding domain 1. Biochimie (19915j0) |
| 2010 | Zifarelli G, Pusch M Relaxing messages from the sarcolemma Journal Of General Physiology (54824j0) |
| 2010 | Zifarelli G, Pusch M The role of protons in fast and slow gating of the Torpedo chloride channel ClC-0. European Biophysics Journal With Biophysics Letters (05881j0) |
| 2009 | De Angeli A, Moran O, Wege S, Filleur S, Ephritikhine G, Thomine S, Barbier-Brygoo H, Gambale F ATP binding to the C-terminus of the Arabidopsis thaliana, AtCLCa, regulates nitrate transport into plant vacuoles. Journal Of Biological Chemistry (51172j0) |
| 2009 | Zifarelli G, Pusch M Conversion of the 2 Cl(-)/1 H(+) antiporter ClC-5 in a NO(3)(-)/H(+) antiporter by a single point mutation. Embo Journal (04123j0) |
| 2009 | Zifarelli G, Pusch M Intracellular regulation of human ClC-5 by adenine nucleotides. Embo Reports (13482j0) |
| 2009 | Pusch M, Zifarelli G It's the proton also in ClC-2. Journal Of Physiology-london (59928j0) |
| 2009 | Gavazzo P, Guida P, Zanardi I, Marchetti C Molecular determinants of multiple effects of Nickel on NMDA receptor channels Neurotoxicity Research (14104) |
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