SiglaMD.P01.008.001
Inizio2009
Termine2018
CommessaMD.P01.008
ResponsabileEdi Gabellieri
Tematiche di ricercaa) Sviluppo di nuove metodologie ed apparati strumentali basati sulla spettroscopia di fluorescenza e fosforescenza del triptofano, idonei per lo studio di proprietà strutturali e dinamiche di proteine in differenti mezzi e condizioni sperimentali (soluzioni acquose, membrane biologiche, ghiaccio, stato secco, micelle, silica gel, film sottili, proteine adsorbite su supporto solido), utilizzando anche variazioni di pressione idrostatica (fino a 7 kbar).
b) Progettazione e realizzazione di sonde ottiche (fluorescenti e fosforescenti) reporter di parametri biomolecolari (polarità, viscosità, presenza di specifici metaboliti) per spettroscopia in vitro ed imaging cellulare ad alta risoluzione spaziale e temporale.
Personale di Ricerca (11)
Nome COGNOMEStrutturaRuolo
Giovanni CercignaniIBF.PIPersonale associato
Patrizia CioniIBF.PIPersonale di ricerca
Giuliano ColombettiIBF.PIPersonale associato
Serena FaggianoIBF.PIPersonale associato
Edi GabellieriIBF.PIPersonale di ricerca
Margherita GonnelliIBF.PIPersonale di ricerca
Francesco LenciIBF.PIPersonale associato
Sabina LuciaIBF.PIPersonale di ricerca
Roberto MarangoniIBF.PIPersonale associato
Andrea MozzarelliIBF.PIPersonale associato
Samanta RaboniIBF.PIPersonale collaboratore
Pubblicazioni (69)
AnnoAutori/Titolo/Rivista
2017Bruno S, Margiotta M, Marchesani F, Paredi G, Orlandi V, Faggiano S, Ronda L, Campanini B, Mozzarelli A
Magnesium and calcium ions differentially affect human serine racemase activity and modulate its quaternary equilibrium toward a tetrameric form
Biochimica Et Biophysica Acta. Proteins And Proteomic
2017Benoni R, De Bei O, Paredi GL, Hayes CS, Franko N, Mozzarelli A, Bettati S, Campanini B
Modulation of Escherichia coli serine acetyltransferase catalytic activity in the cysteine synthase complex
Febs Letters (print
2016Jacchetti E.; Gabellieri E.; Cioni P.; Bizzarri R.; Nifosi R.
Temperature and pressure effects on GFP mutants: Explaining spectral changes by molecular dynamics simulations and TD-DFT calculations
Pccp. Physical Chemistry Chemical Physics (print)
2016Benoni R, Pertinhez TA, Spyrakis F, Davalli S, Pellegrino S, Paredi GL, Pezzotti A, Bettati S, Campanini B, Mozzarelli A
Structural insight into the interaction of O-acetylserine sulfhydrylase with competitive, peptidic inhibitors by saturation transfer difference-NMR
Febs Letters (print)
2016Beato C, Pecchini C, Cocconcelli C, Campanini B, Marchetti ML, Pieroni M, Mozzarelli A, Costantino G
Cyclopropane derivatives as potential human serine racemase inhibitors: unveiling novel insights into a difficult target
Journal Of Enzyme Inhibition And Medicinal Chemistry (print)
2016Annunziato G, Pieroni M, Benoni R, Campanini B, Pertinhez TA, Pecchini C, Bruno A, Magalhães J, Bettati S, Franko N, Mozzarelli A, Costantino G
Cyclopropane-1,2-dicarboxylic acids as new tools for the biophysical investigation of O-acetylserine sulfhydrylases by fluorimetric methods and saturation transfer difference (STD) NMR
Journal Of Enzyme Inhibition And Medicinal Chemistry (print)
2016Cavallini G.; Sgarbossa A.; Parentini I.; Bizzarri R.; Donati A.; Lenci F.; Bergamini E.
Dolichol: A Component of the Cellular Antioxidant Machinery
Lipids
2016Paredi G, Raboni S, Marchesani F, Ordoudi SA, Tsimidou MZ, Mozzarelli A
Insight of Saffron Proteome by Gel-Electrophoresis
Molecules (basel, Online)
2016Bruno S, Margiotta M, Pinto A, Cullia G, Conti P, De Micheli C, Mozzarelli A
Selectivity of 3-bromo-isoxazoline inhibitors between human and Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenases
Bioorganic & Medicinal Chemistry (print)
2016Pieroni M, Annunziato G, Beato C, Wouters R, Benoni R, Campanini B, Pertinhez TA, Bettati S, Mozzarelli A, Costantino G
Rational Design, Synthesis, and Preliminary Structure-Activity Relationships of alpha-Substituted-2-Phenylcyclopropane Carboxylic Acids as Inhibitors of Salmonella typhimurium O-Acetylserine Sulfhydrylase
Journal Of Medicinal Chemistry
2015Beato C, Pecchini C, Cocconcelli C, Campanini B, Marchetti ML, Pieroni M, Mozzarelli A, Costantino G
Cyclopropane derivatives as potential human serine racemase inhibitors: unveiling novel insights into a difficult target
Journal Of Enzyme Inhibition And Medicinal Chemistry (print)
2015Mavelli F.; Marangoni R.; Stano P.
A Simple Protein Synthesis Model for the PURE System Operation
Bulletin Of Mathematical Biology (print)
2015Dellafiora L.; Marchetti M.; Spyrakis F.; Orlandi V.; Campanini B.; Cruciani G.; Cozzini P.; Mozzarelli A.
Expanding the chemical space of human serine racemase inhibitors
Bioorganic & Medicinal Chemistry Letters (print)
2015Henry E.R.; Mozzarelli A.; Viappiani C.; Abbruzzetti S.; Bettati S.; Ronda L.; Bruno S.; Eaton W.A.
Experiments on Hemoglobin in Single Crystals and Silica Gels Distinguish among Allosteric Models
Biophysical Journal (print)
2015Ronda, Luca; Bruno, Stefano; Bettati, Stefano; Storici, Paola; Mozzarelli, Andrea
From protein structure to function via single crystal optical spectroscopy
Frontiers In Molecular Biosciences
2015Ronda L.[ 1 ]; Bruno S.[ 2 ]; Campanini B.[ 2 ]; Mozzarelli A.[ 2,6,7 ]; Abbruzzetti S.[ 3,8 ]; Viappiani C.[ 4,8 ] ; Cupane A.[ 5 ] ; Levantino M.[ 5 ] ; Bettati S.[ 1,6 ]
Immobilization of proteins in silica gel: Biochemical and biophysical properties
Current Organic Chemistry
2015Morelli E., Salvadori E., Basso B., Tognotti D., Cioni P., Gabellieri E.
The response of Phaeodactylum tricornutum to quantum dot exposure: Acclimation and changes in protein expression.
Marine Environmental Research
2015Fulgentini L.; Passini V.; Colombetti G.; Miceli C.; La Terza A.; Marangoni R.
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii
Microbial Ecology
2014Retelletti Brogi S.; Gonnelli M.; Vestri S.; Santinelli C.
Biophysical processes affecting DOM dynamics at the Arno river mouth (Tyrrhenian Sea)
Biophysical Chemistry (print)
2014Caltagirone, Claudia; Falchi, Angela Maria; Lampis, Sandrina; Lippolis, Vito; Meli, Valeria; Monduzzi, Maura; Prodi, Luca; Schmidt, Judith; Sgarzi, Massimo; Talmon, Yeshayahu; Bizzarri, Ranieri; Murgia, Sergio
Cancer-Cell-Targeted Theranostic Cubosomes
Langmuir
2014Bianchini P.[ 1 ]; Cardarelli F.[ 2 ]; Di Luca M.[ 3,4 ]; Diaspro A.[ 1 ]; Bizzarri R.[ 1,3,4,5 ]
Nanoscale protein diffusion by STED-based pair correlation analysis
Plos One
2014Prescimone T.; Tognotti D.; Caselli C.; Cabiati M.; D'Amico A.; Del Ry S.; Giannessi D.
Reappraisal of Quantitative Gel Zymography for Matrix Metalloproteinases
Journal Of Clinical Laboratory Analysis (print)
2014Cioni P.; Gabellieri E.; Marchal S.; Lange R.
Temperature and pressure effects on C112S azurin: Volume, expansivity, and flexibility changes
Proteins (online)
2013Signore G. [ 1 ] ; Abbandonato G. [ 1,2,3 ] ; Storti B. [ 1,2,3 ] ; Stockl M. [ 4,5 ] ; Subramaniam V.[ 4,5 ] ; Bizzarri R. [ 2,3,6 ]
Imaging the static dielectric constant in vitro and in living cells by a bioconjugable GFP chromophore analog
Chemical Communications
2013Morelli E, Salvadori E, Bizzarri R, Cioni P, Gabellieri E
Interaction of CdSe/ZnS quantum dots with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta: A biophysical approach
Biophysical Chemistry
2013Shalit A. [ 1 ] ; Lucchetta D. E. [ 2 ] ; Criante L. [ 2,3 ] ; Vita F. [ 2 ] ; Tasseva J. R. [ 4,5,6 ] ; Simoni F. [ 2 ] ; Franco L. [ 7 ] ; Bizzarri R. [ 8 ] ; Faraci P. [ 9 ] ; Conte R. [ 10,11 ] ; Viti L. [ 12 ] ; Kanera R. [ 1 ] ; Castagna R. [ 1,12 ]
Laser-Light Polarization Plastic Visualizer: Light-Scattering Distribution and Anisotropy
Rsc Advances
2013Tognotti D, Gabellieri E, Morelli E, Cioni P
Temperature and pressure dependence of azurin stability as monitored by tryptophan fluorescence and phosphorescence. The case of F29A mutant
Biophysical Chemistry
2013Antonella Sgarbossa; Susanna Monti; Francesco Lenci; Emilia Bramanti; Ranieri Bizzarri; Vincenzo Barone
The Effects of Ferulic Acid on beta-Amyloid Fibrillar Structures Investigated through Experimental and Computational Techniques
Biochimica Et Biophysica Acta. G, General Subjects
2013Gonnelli M, Vestri S, Santinelli C
Chromophoric dissolved organic matter and microbial enzymatic activity. A biophysical approach to understand the marine carbon cycle
Biophysical Chemistry
2013Bramanti E, Fulgentini L, Bizzarri R, Lenci F, Sgarbossa A
beta-Amyloid Amorphous Aggregates Induced by the Small Natural Molecule Ferulic Acid
The Journal Of Physical Chemistry
2012Giansoldati V; Tassi E; Morelli E; Gabellieri E; Pedron F; Barbafieri M
Nitrogen fertilizer improves boron phytoextraction by Brassica juncea grown in contaminated sediments and alleviates plant stress
Chemosphere
2012Morelli, E ; Cioni, P ; Posarelli, M; Gabellieri, E
Chemical stability of CdSe quantum dots in seawater and their effects on a marine microalga
Aquatic Toxicology
2012A. Shalit, D. E. Lucchetta, V. Piazza, F. Simoni, R. Bizzarri, R. Castagna
Polarization-dependent laser-light structured directionality with polymer composite materials
Materials Letters
2011Strambini GB, Gonnelli M
Amplitude spectrum of structural fluctuations in proteins from the internal diffusion of solutes of increasing molecular size: a Trp phosphorescence quenching study
Biochemistry
2011Gabellieri E, Bucciantini M, Stefani M, Cioni P
Does azurin bind to the transactivation domain of p53? A Trp phosphorescence study
Biophysical Chemistry
2011Strambini GB, Gonnelli M
Influence of Denaturants on Native-State Structural Fluctuations in Azurin Probed by Molecular Size-Dependent Quenching of Trp Phosphorescence
Journal Of Physical Chemistry B
2011Cioni P, Gabellieri E
Protein dynamics and pressure: what can high pressure tell us about protein structural flexibility?
Biochimica Et Biophysica Acta-proteins And Proteomics
2011Gabellieri E, Cioni P, Balestreri E, Morelli E
Protein structural changes induced by glutathione-coated CdS quantum dots as revealed by Trp phosphorescence
European Biophysics Journal With Biophysics Letters
2010Strambini GB, Gonnelli M
Acrylonitrile quenching of trp phosphorescence in proteins: a probe of the internal flexibility of the globular fold.
Biophysical Journal
2010Strambini GB, Gonnelli M
Fluorescence quenching of buried Trp residues by acrylamide does not require penetration of the protein fold.
Journal Of Physical Chemistry B
2010Strambini GB, Gonnelli M
Protein phosphorescence quenching: distinction between quencher penetration and external quenching mechanisms.
Journal Of Physical Chemistry B
2009Strambini GB, Gonnelli M
Acrylamide quenching of Trp phosphorescence in liver alcohol dehydrogenase: evidence of gated quencher penetration.
Biochemistry
2009Gonnelli M, Strambini GB
No effect of covalently linked poly(ethylene glycol) chains on protein internal dynamics
Biochimica Et Biophysica Acta-proteins And Proteomics
2009Turchi G, Alagona G, Lubrano V
Protective activity of plicatin B against human LDL oxidation induced in metal ion-dependent and -independent processes. Experimental and theoretical studies.
Phytomedicine
2009Eventi A, Lubrano V, Scarpato R, Turchi G
Protective effects of plicatin B on micronucleus induction in cultured human lymphocytes by different mutagens
Food And Chemical Toxicology
2009Donadio E, Piccolomini F, Dimuccio V, Felicioli A, Balestreri E, Cianti R, Armini A, Bini L, Felicioli R, Donadio C
Serum albumin fragmentation in end-stage renal disease patients – a pilot study.
Clinical Chemistry And Laboratory Medicine
2009D'Auria S, Aurilia V, Marabotti A, Gonnelli M, Strambini G
Structure and dynamics of cold-adapted enzymes as investigated by phosphorescence spectroscopy and molecular dynamics studies. 2. The case of an esterase from Pseudoalteromonas haloplanktis.
Journal Of Physical Chemistry B
2008Gabellieri E, Balestreri E, Galli A., and P Cioni.
Cavity-Creating Mutations in Pseudomonas aeruginosa Azurin: Effects on Protein Dynamics and Stability.
Biophysical Journal (print)
2008Coltelli P., Evangelisti M., Evangelista V., Gualtieri P.
An automatic real-time system for the determination of translational and rotational speeds of swimming micro-organisms
International Journal Of Signal And Imaging Systems Engineering (print)
2008Kerwin BA, Aoki KH, Gonelli M, Strambini GB.
Differentiation of the Local Structure Around Tryptophan 51 and 64 in Recombinant Human Erythropoietin by Tryptophan Phosphorescence.
Photochemistry And Photobiology
2008Strambini GB, Balestreri E, Galli A, Gonnelli M.
Effects of sugars and polyols on the stability of azurin in ice.
The Journal Of Physical Chemistry. B
2008Juarez AB, Barsanti L., Evangelista V., Passarelli V., Vesentini N., Conforti V., and Gualtieri P
In vivo microspectroscopy monitoring of Chromium Effects on the photosynthetic and photoreceptive apparatus of Chlorella kessleri and Eudorina unicocca
Journal Of Environmental Monitoring (print)
2008Vesentini N.; Kusmic C.; Battaglia D.; Taddei M. C.; Barsanti L.; Parodi O.; Palombo C.; Paolicchi A.; Barsacchi R.
Modulation of erythrocyte sensitivity to oxidative stress by transient hyperhomocysteinemia in healthy subjects and in patients with coronary artery disease.
Nmcd. Nutrition Metabolism And Cardiovascular Diseases (testo Stamp.)
2008Tveen Jensen K; Strambini GB; Gonnelli M; Hesp BH; Broos J and Jackson JB
Mutations in Tranhydrogenase Change The Fluorescence Emission State of Trp-72 From 1La to 1Lb
Biophysical Journal (print)
2008Strambini GB; Gonnelli M.
Singular efficacy of trimethylamine N-oxide to counter protein destabilization in ice.
Biochemistry (easton)
2008Strambini GB; Gonnelli M.
Specific Anions Effects of on the Stability of Azurin in Ice.
The Journal Of Physical Chemistry. B
2008D'Auria S., Staiano M., Varriale A., Gonnelli M., Marabotti A., Rossi M., and Strambini G.B.
The differences in the microenvironment of the two tryptophan residues of the glutamine-binding protein from Escherichia coli shed light on the binding properties and the structural dynamics of the protein.
Proteins (print)
2008D'Auria S.; Staiano M.; Varriale A.; Gonnelli M.; Marabotti A.; Rossi M.; Strambini GB.
The tryptophan phosphorescence of porcine and mutant bovine odorant-binding proteins: a probe for the local protein structure and dynamics
Journal Of Proteome Research (print)
2007Lubrano V., Boni G., L?Abbate A., Turchi G
Antioxidant activity of plicatin B on cultured human microvascular endothelial cells exposed to H2O2
Drug And Chemical Toxicology
2007Strambini GB, Gonnelli M.
Protein stability in ice
Biophysical Journal
2007D'Auria S.; Varriale A.; Gonnelli M.; Saviano M.; Staiano M.; Rossi M.; Strambini G.B.
Tryptophan phosphorescence studies of the D-galactose/D-glucose-binding protein from Escherichia coli provide a molecular portrait with structural and dynamics features of the protein
Journal Of Proteome Research
2007Evangelista V, Evangelisti M, Barsanti L, Frassanito AM, Passarelli V, Gualtieri P.
A polychromator-based microspectrophotometer.
International Journal Of Biological Sciences
2007Barsanti L., Evangelista V., Frassanito A.M., Vesentini N., Passarelli V., Gualtieri P.
Absorption microspectroscopy, theory and applications in the case of the photosynthetic compartment.
Micron
2007Coltelli P., Evangelisti M., Evangelista V., Gualtieri P.
An automatic real-time system for the determination of translational and rotational speeds of swimming micro-organisms.
2006Cioni P.
Role of protein cavities on unfolding volume change and on internal dynamics under pressure.
Biophysical Journal (print)
2006Evangelista V., Frassanito A.M., Passarelli V., Barsanti L., Gualtieri P.
Microspectroscopy of the photosynthetic compartment of algae.
Photochemistry And Photobiology
2006Gabellieri E., G.B. Strambini, D. Shcharbin, B. Klainert, M. Bryszewska
Dendrimer-Protein Interactions Studied by Tryptophan Room Temperature Phosphorescence
Biochimica Et Biophysica Acta. Proteins And Proteomics
2006Gabellieri E., Strambini G.
ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in Ice
Biophysical Journal (print)
2006Piccolomini F.; Wratten M.L.; Sereni L.; Balestreri E.; Donadio E.; Felicioli A.; Felicioli R.; Donadio C.
Effects of the incubation in vitro with sorbents on serum proteomic pattern and cytokine concentration in cancer patients during chemotherapy-preliminary results.
Biomedicine & Pharmacotherapy